Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer
Neutralizing antibody (NAb) titer is a key biomarker of protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, but point-of-care methods for assessing NAb titer are not widely available. Here, we present a lateral flow assay that captures SARS-CoV-2 receptor-bindi...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2023
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| Online Access: | https://hdl.handle.net/1721.1/147025 |
| _version_ | 1826200629151793152 |
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| author | Connelly, Guinevere G Kirkland, Orville O Bohannon, Seven Lim, Daniel C Wilson, Robert M Richards, Edward J Tay, Dousabel M Jee, Hyuk Hellinger, Riley D Hoang, Ngoc K Hao, Liang Chhabra, Arnav Martin-Alonso, Carmen Tan, Edward KW Koehler, Angela N Yaffe, Michael B London, Wendy B Lee, Pui Y Krammer, Florian Bohannon, Robert C Bhatia, Sangeeta N Sikes, Hadley D Li, Hojun |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Connelly, Guinevere G Kirkland, Orville O Bohannon, Seven Lim, Daniel C Wilson, Robert M Richards, Edward J Tay, Dousabel M Jee, Hyuk Hellinger, Riley D Hoang, Ngoc K Hao, Liang Chhabra, Arnav Martin-Alonso, Carmen Tan, Edward KW Koehler, Angela N Yaffe, Michael B London, Wendy B Lee, Pui Y Krammer, Florian Bohannon, Robert C Bhatia, Sangeeta N Sikes, Hadley D Li, Hojun |
| author_sort | Connelly, Guinevere G |
| collection | MIT |
| description | Neutralizing antibody (NAb) titer is a key biomarker of protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, but point-of-care methods for assessing NAb titer are not widely available. Here, we present a lateral flow assay that captures SARS-CoV-2 receptor-binding domain (RBD) that has been neutralized from binding angiotensin-converting enzyme 2 (ACE2). Quantification of neutralized RBD in this assay correlates with NAb titer from vaccinated and convalescent patients. This methodology demonstrated superior performance in assessing NAb titer compared with either measurement of total anti-spike immunoglobulin G titer or quantification of the absolute reduction in binding between ACE2 and RBD. Our testing platform has the potential for mass deployment to aid in determining at population scale the degree of protective immunity individuals may have following SARS-CoV-2 vaccination or infection and can enable simple at-home assessment of NAb titer. |
| first_indexed | 2024-09-23T11:39:23Z |
| format | Article |
| id | mit-1721.1/147025 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:39:23Z |
| publishDate | 2023 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1470252023-01-10T03:29:00Z Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer Connelly, Guinevere G Kirkland, Orville O Bohannon, Seven Lim, Daniel C Wilson, Robert M Richards, Edward J Tay, Dousabel M Jee, Hyuk Hellinger, Riley D Hoang, Ngoc K Hao, Liang Chhabra, Arnav Martin-Alonso, Carmen Tan, Edward KW Koehler, Angela N Yaffe, Michael B London, Wendy B Lee, Pui Y Krammer, Florian Bohannon, Robert C Bhatia, Sangeeta N Sikes, Hadley D Li, Hojun Massachusetts Institute of Technology. Department of Biology Neutralizing antibody (NAb) titer is a key biomarker of protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, but point-of-care methods for assessing NAb titer are not widely available. Here, we present a lateral flow assay that captures SARS-CoV-2 receptor-binding domain (RBD) that has been neutralized from binding angiotensin-converting enzyme 2 (ACE2). Quantification of neutralized RBD in this assay correlates with NAb titer from vaccinated and convalescent patients. This methodology demonstrated superior performance in assessing NAb titer compared with either measurement of total anti-spike immunoglobulin G titer or quantification of the absolute reduction in binding between ACE2 and RBD. Our testing platform has the potential for mass deployment to aid in determining at population scale the degree of protective immunity individuals may have following SARS-CoV-2 vaccination or infection and can enable simple at-home assessment of NAb titer. 2023-01-09T18:34:28Z 2023-01-09T18:34:28Z 2022 2023-01-09T18:28:12Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147025 Connelly, Guinevere G, Kirkland, Orville O, Bohannon, Seven, Lim, Daniel C, Wilson, Robert M et al. 2022. "Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer." Cell Reports Methods, 2 (8). en 10.1016/J.CRMETH.2022.100273 Cell Reports Methods Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV Elsevier |
| spellingShingle | Connelly, Guinevere G Kirkland, Orville O Bohannon, Seven Lim, Daniel C Wilson, Robert M Richards, Edward J Tay, Dousabel M Jee, Hyuk Hellinger, Riley D Hoang, Ngoc K Hao, Liang Chhabra, Arnav Martin-Alonso, Carmen Tan, Edward KW Koehler, Angela N Yaffe, Michael B London, Wendy B Lee, Pui Y Krammer, Florian Bohannon, Robert C Bhatia, Sangeeta N Sikes, Hadley D Li, Hojun Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer |
| title | Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer |
| title_full | Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer |
| title_fullStr | Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer |
| title_full_unstemmed | Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer |
| title_short | Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer |
| title_sort | direct capture of neutralized rbd enables rapid point of care assessment of sars cov 2 neutralizing antibody titer |
| url | https://hdl.handle.net/1721.1/147025 |
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