A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex
<jats:title>Abstract</jats:title><jats:p>Single-cell transcriptomics can provide quantitative molecular signatures for large, unbiased samples of the diverse cell types in the brain<jats:sup>1–3</jats:sup>. With the proliferation of multi-omics datasets, a major challen...
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2023
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| Online Access: | https://hdl.handle.net/1721.1/147091 |
| _version_ | 1826216507101675520 |
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| author | Regev, Aviv |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Regev, Aviv |
| author_sort | Regev, Aviv |
| collection | MIT |
| description | <jats:title>Abstract</jats:title><jats:p>Single-cell transcriptomics can provide quantitative molecular signatures for large, unbiased samples of the diverse cell types in the brain<jats:sup>1–3</jats:sup>. With the proliferation of multi-omics datasets, a major challenge is to validate and integrate results into a biological understanding of cell-type organization. Here we generated transcriptomes and epigenomes from more than 500,000 individual cells in the mouse primary motor cortex, a structure that has an evolutionarily conserved role in locomotion. We developed computational and statistical methods to integrate multimodal data and quantitatively validate cell-type reproducibility. The resulting reference atlas—containing over 56 neuronal cell types that are highly replicable across analysis methods, sequencing technologies and modalities—is a comprehensive molecular and genomic account of the diverse neuronal and non-neuronal cell types in the mouse primary motor cortex. The atlas includes a population of excitatory neurons that resemble pyramidal cells in layer 4 in other cortical regions<jats:sup>4</jats:sup>. We further discovered thousands of concordant marker genes and gene regulatory elements for these cell types. Our results highlight the complex molecular regulation of cell types in the brain and will directly enable the design of reagents to target specific cell types in the mouse primary motor cortex for functional analysis.</jats:p> |
| first_indexed | 2024-09-23T16:48:28Z |
| format | Article |
| id | mit-1721.1/147091 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:48:28Z |
| publishDate | 2023 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1470912023-01-14T03:01:28Z A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex Regev, Aviv Massachusetts Institute of Technology. Department of Biology <jats:title>Abstract</jats:title><jats:p>Single-cell transcriptomics can provide quantitative molecular signatures for large, unbiased samples of the diverse cell types in the brain<jats:sup>1–3</jats:sup>. With the proliferation of multi-omics datasets, a major challenge is to validate and integrate results into a biological understanding of cell-type organization. Here we generated transcriptomes and epigenomes from more than 500,000 individual cells in the mouse primary motor cortex, a structure that has an evolutionarily conserved role in locomotion. We developed computational and statistical methods to integrate multimodal data and quantitatively validate cell-type reproducibility. The resulting reference atlas—containing over 56 neuronal cell types that are highly replicable across analysis methods, sequencing technologies and modalities—is a comprehensive molecular and genomic account of the diverse neuronal and non-neuronal cell types in the mouse primary motor cortex. The atlas includes a population of excitatory neurons that resemble pyramidal cells in layer 4 in other cortical regions<jats:sup>4</jats:sup>. We further discovered thousands of concordant marker genes and gene regulatory elements for these cell types. Our results highlight the complex molecular regulation of cell types in the brain and will directly enable the design of reagents to target specific cell types in the mouse primary motor cortex for functional analysis.</jats:p> 2023-01-13T13:55:26Z 2023-01-13T13:55:26Z 2021 2023-01-13T13:48:49Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147091 Regev, Aviv. 2021. "A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex." Nature, 598 (7879). en 10.1038/S41586-021-03500-8 Nature Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Regev, Aviv A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex |
| title | A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex |
| title_full | A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex |
| title_fullStr | A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex |
| title_full_unstemmed | A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex |
| title_short | A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex |
| title_sort | transcriptomic and epigenomic cell atlas of the mouse primary motor cortex |
| url | https://hdl.handle.net/1721.1/147091 |
| work_keys_str_mv | AT regevaviv atranscriptomicandepigenomiccellatlasofthemouseprimarymotorcortex AT regevaviv transcriptomicandepigenomiccellatlasofthemouseprimarymotorcortex |