Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium
<jats:title>Abstract</jats:title><jats:p>The inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily b...
| Main Authors: | , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2023
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| Online Access: | https://hdl.handle.net/1721.1/147101 |
| _version_ | 1811093620518813696 |
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| author | Vickovic, Sanja Schapiro, Denis Carlberg, Konstantin Lötstedt, Britta Larsson, Ludvig Hildebrandt, Franziska Korotkova, Marina Hensvold, Aase H Catrina, Anca I Sorger, Peter K Malmström, Vivianne Regev, Aviv Ståhl, Patrik L |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Vickovic, Sanja Schapiro, Denis Carlberg, Konstantin Lötstedt, Britta Larsson, Ludvig Hildebrandt, Franziska Korotkova, Marina Hensvold, Aase H Catrina, Anca I Sorger, Peter K Malmström, Vivianne Regev, Aviv Ståhl, Patrik L |
| author_sort | Vickovic, Sanja |
| collection | MIT |
| description | <jats:title>Abstract</jats:title><jats:p>The inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily been studied either by immunostaining or by molecular profiling after tissue homogenization. Here, we use Spatial Transcriptomics, where tissue-resident RNA is spatially labeled in situ with barcodes in a transcriptome-wide fashion, to study local tissue interactions at the site of chronic synovial inflammation. We report comprehensive spatial RNA-Seq data coupled to cell type-specific localization patterns at and around organized structures of infiltrating leukocyte cells in the synovium. Combining morphological features and high-throughput spatially resolved transcriptomics may be able to provide higher statistical power and more insights into monitoring disease severity and treatment-specific responses in seropositive and seronegative rheumatoid arthritis.</jats:p> |
| first_indexed | 2024-09-23T15:48:00Z |
| format | Article |
| id | mit-1721.1/147101 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:48:00Z |
| publishDate | 2023 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1471012023-01-14T03:05:09Z Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium Vickovic, Sanja Schapiro, Denis Carlberg, Konstantin Lötstedt, Britta Larsson, Ludvig Hildebrandt, Franziska Korotkova, Marina Hensvold, Aase H Catrina, Anca I Sorger, Peter K Malmström, Vivianne Regev, Aviv Ståhl, Patrik L Massachusetts Institute of Technology. Department of Biology <jats:title>Abstract</jats:title><jats:p>The inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily been studied either by immunostaining or by molecular profiling after tissue homogenization. Here, we use Spatial Transcriptomics, where tissue-resident RNA is spatially labeled in situ with barcodes in a transcriptome-wide fashion, to study local tissue interactions at the site of chronic synovial inflammation. We report comprehensive spatial RNA-Seq data coupled to cell type-specific localization patterns at and around organized structures of infiltrating leukocyte cells in the synovium. Combining morphological features and high-throughput spatially resolved transcriptomics may be able to provide higher statistical power and more insights into monitoring disease severity and treatment-specific responses in seropositive and seronegative rheumatoid arthritis.</jats:p> 2023-01-13T16:55:38Z 2023-01-13T16:55:38Z 2022 2023-01-13T15:35:14Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147101 Vickovic, Sanja, Schapiro, Denis, Carlberg, Konstantin, Lötstedt, Britta, Larsson, Ludvig et al. 2022. "Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium." Communications Biology, 5 (1). en 10.1038/S42003-022-03050-3 Communications Biology Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Vickovic, Sanja Schapiro, Denis Carlberg, Konstantin Lötstedt, Britta Larsson, Ludvig Hildebrandt, Franziska Korotkova, Marina Hensvold, Aase H Catrina, Anca I Sorger, Peter K Malmström, Vivianne Regev, Aviv Ståhl, Patrik L Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium |
| title | Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium |
| title_full | Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium |
| title_fullStr | Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium |
| title_full_unstemmed | Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium |
| title_short | Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium |
| title_sort | three dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium |
| url | https://hdl.handle.net/1721.1/147101 |
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