Pooled genetic screens with image‐based profiling
Spatial structure in biology, spanning molecular, organellular, cellular, tissue, and organismal scales, is encoded through a combination of genetic and epigenetic factors in individual cells. Microscopy remains the most direct approach to exploring the intricate spatial complexity defining biologic...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
EMBO
2023
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| Online Access: | https://hdl.handle.net/1721.1/147777 |
| _version_ | 1826201243532394496 |
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| author | Walton, Russell T Singh, Avtar Blainey, Paul C |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Walton, Russell T Singh, Avtar Blainey, Paul C |
| author_sort | Walton, Russell T |
| collection | MIT |
| description | Spatial structure in biology, spanning molecular, organellular, cellular, tissue, and organismal scales, is encoded through a combination of genetic and epigenetic factors in individual cells. Microscopy remains the most direct approach to exploring the intricate spatial complexity defining biological systems and the structured dynamic responses of these systems to perturbations. Genetic screens with deep single-cell profiling via image features or gene expression programs have the capacity to show how biological systems work in detail by cataloging many cellular phenotypes with one experimental assay. Microscopy-based cellular profiling provides information complementary to next-generation sequencing (NGS) profiling and has only recently become compatible with large-scale genetic screens. Optical screening now offers the scale needed for systematic characterization and is poised for further scale-up. We discuss how these methodologies, together with emerging technologies for genetic perturbation and microscopy-based multiplexed molecular phenotyping, are powering new approaches to reveal genotype-phenotype relationships. |
| first_indexed | 2024-09-23T11:48:33Z |
| format | Article |
| id | mit-1721.1/147777 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:48:33Z |
| publishDate | 2023 |
| publisher | EMBO |
| record_format | dspace |
| spelling | mit-1721.1/1477772023-02-01T03:34:00Z Pooled genetic screens with image‐based profiling Walton, Russell T Singh, Avtar Blainey, Paul C Massachusetts Institute of Technology. Department of Biological Engineering Spatial structure in biology, spanning molecular, organellular, cellular, tissue, and organismal scales, is encoded through a combination of genetic and epigenetic factors in individual cells. Microscopy remains the most direct approach to exploring the intricate spatial complexity defining biological systems and the structured dynamic responses of these systems to perturbations. Genetic screens with deep single-cell profiling via image features or gene expression programs have the capacity to show how biological systems work in detail by cataloging many cellular phenotypes with one experimental assay. Microscopy-based cellular profiling provides information complementary to next-generation sequencing (NGS) profiling and has only recently become compatible with large-scale genetic screens. Optical screening now offers the scale needed for systematic characterization and is poised for further scale-up. We discuss how these methodologies, together with emerging technologies for genetic perturbation and microscopy-based multiplexed molecular phenotyping, are powering new approaches to reveal genotype-phenotype relationships. 2023-01-30T14:52:04Z 2023-01-30T14:52:04Z 2022-11 2023-01-30T14:06:27Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147777 Walton, Russell T, Singh, Avtar and Blainey, Paul C. 2022. "Pooled genetic screens with image‐based profiling." Molecular Systems Biology, 18 (11). en 10.15252/msb.202110768 Molecular Systems Biology Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf EMBO EMBO Press |
| spellingShingle | Walton, Russell T Singh, Avtar Blainey, Paul C Pooled genetic screens with image‐based profiling |
| title | Pooled genetic screens with image‐based profiling |
| title_full | Pooled genetic screens with image‐based profiling |
| title_fullStr | Pooled genetic screens with image‐based profiling |
| title_full_unstemmed | Pooled genetic screens with image‐based profiling |
| title_short | Pooled genetic screens with image‐based profiling |
| title_sort | pooled genetic screens with image based profiling |
| url | https://hdl.handle.net/1721.1/147777 |
| work_keys_str_mv | AT waltonrussellt pooledgeneticscreenswithimagebasedprofiling AT singhavtar pooledgeneticscreenswithimagebasedprofiling AT blaineypaulc pooledgeneticscreenswithimagebasedprofiling |