Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging
<jats:p> Animal genomes are folded into loops and topologically associating domains (TADs) by CTCF and loop-extruding cohesins, but the live dynamics of loop formation and stability remain unknown. Here, we directly visualized chromatin looping at the <jats:italic&...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2023
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| Online Access: | https://hdl.handle.net/1721.1/147831 |
| _version_ | 1811098074794164224 |
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| author | Gabriele, Michele Brandão, Hugo B Grosse-Holz, Simon Jha, Asmita Dailey, Gina M Cattoglio, Claudia Hsieh, Tsung-Han S Mirny, Leonid Zechner, Christoph Hansen, Anders S |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Gabriele, Michele Brandão, Hugo B Grosse-Holz, Simon Jha, Asmita Dailey, Gina M Cattoglio, Claudia Hsieh, Tsung-Han S Mirny, Leonid Zechner, Christoph Hansen, Anders S |
| author_sort | Gabriele, Michele |
| collection | MIT |
| description | <jats:p>
Animal genomes are folded into loops and topologically associating domains (TADs) by CTCF and loop-extruding cohesins, but the live dynamics of loop formation and stability remain unknown. Here, we directly visualized chromatin looping at the
<jats:italic>Fbn2</jats:italic>
TAD in mouse embryonic stem cells using super-resolution live-cell imaging and quantified looping dynamics by Bayesian inference. Unexpectedly, the
<jats:italic>Fbn2</jats:italic>
loop was both rare and dynamic, with a looped fraction of approximately 3 to 6.5% and a median loop lifetime of approximately 10 to 30 minutes. Our results establish that the
<jats:italic>Fbn2</jats:italic>
TAD is highly dynamic, and about 92% of the time, cohesin-extruded loops exist within the TAD without bridging both CTCF boundaries. This suggests that single CTCF boundaries, rather than the fully CTCF-CTCF looped state, may be the primary regulators of functional interactions.
</jats:p> |
| first_indexed | 2024-09-23T17:09:30Z |
| format | Article |
| id | mit-1721.1/147831 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T17:09:30Z |
| publishDate | 2023 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/1478312023-02-02T03:00:27Z Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging Gabriele, Michele Brandão, Hugo B Grosse-Holz, Simon Jha, Asmita Dailey, Gina M Cattoglio, Claudia Hsieh, Tsung-Han S Mirny, Leonid Zechner, Christoph Hansen, Anders S Massachusetts Institute of Technology. Department of Biological Engineering <jats:p> Animal genomes are folded into loops and topologically associating domains (TADs) by CTCF and loop-extruding cohesins, but the live dynamics of loop formation and stability remain unknown. Here, we directly visualized chromatin looping at the <jats:italic>Fbn2</jats:italic> TAD in mouse embryonic stem cells using super-resolution live-cell imaging and quantified looping dynamics by Bayesian inference. Unexpectedly, the <jats:italic>Fbn2</jats:italic> loop was both rare and dynamic, with a looped fraction of approximately 3 to 6.5% and a median loop lifetime of approximately 10 to 30 minutes. Our results establish that the <jats:italic>Fbn2</jats:italic> TAD is highly dynamic, and about 92% of the time, cohesin-extruded loops exist within the TAD without bridging both CTCF boundaries. This suggests that single CTCF boundaries, rather than the fully CTCF-CTCF looped state, may be the primary regulators of functional interactions. </jats:p> 2023-02-01T17:05:13Z 2023-02-01T17:05:13Z 2022 2023-02-01T16:58:06Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147831 Gabriele, Michele, Brandão, Hugo B, Grosse-Holz, Simon, Jha, Asmita, Dailey, Gina M et al. 2022. "Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging." Science, 376 (6592). en 10.1126/SCIENCE.ABN6583 Science Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Association for the Advancement of Science (AAAS) PMC |
| spellingShingle | Gabriele, Michele Brandão, Hugo B Grosse-Holz, Simon Jha, Asmita Dailey, Gina M Cattoglio, Claudia Hsieh, Tsung-Han S Mirny, Leonid Zechner, Christoph Hansen, Anders S Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging |
| title | Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging |
| title_full | Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging |
| title_fullStr | Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging |
| title_full_unstemmed | Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging |
| title_short | Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging |
| title_sort | dynamics of ctcf and cohesin mediated chromatin looping revealed by live cell imaging |
| url | https://hdl.handle.net/1721.1/147831 |
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