Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis
<jats:p>Cell mass and composition change with cell cycle progression. Our previous work characterized buoyant mass dynamics in mitosis (Miettinen et al., 2019), but how dry mass and cell composition change in mitosis has remained unclear. To better understand mitotic cell growth and compositio...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications, Ltd
2023
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| Online Access: | https://hdl.handle.net/1721.1/147877 |
| _version_ | 1811071024260710400 |
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| author | Miettinen, Teemu P Ly, Kevin S Lam, Alice Manalis, Scott R |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Miettinen, Teemu P Ly, Kevin S Lam, Alice Manalis, Scott R |
| author_sort | Miettinen, Teemu P |
| collection | MIT |
| description | <jats:p>Cell mass and composition change with cell cycle progression. Our previous work characterized buoyant mass dynamics in mitosis (Miettinen et al., 2019), but how dry mass and cell composition change in mitosis has remained unclear. To better understand mitotic cell growth and compositional changes, we develop a single-cell approach for monitoring dry mass and the density of that dry mass every ~75 s with 1.3% and 0.3% measurement precision, respectively. We find that suspension grown mammalian cells lose dry mass and increase dry mass density following mitotic entry. These changes display large, non-genetic cell-to-cell variability, and the changes are reversed at metaphase-anaphase transition, after which dry mass continues accumulating. The change in dry mass density causes buoyant and dry mass to differ specifically in early mitosis, thus reconciling existing literature on mitotic cell growth. Mechanistically, cells in early mitosis increase lysosomal exocytosis, and inhibition of lysosomal exocytosis decreases the dry mass loss and dry mass density increase in mitosis. Overall, our work provides a new approach for monitoring single-cell dry mass and dry mass density, and reveals that mitosis is coupled to extensive exocytosis-mediated secretion of cellular contents.</jats:p> |
| first_indexed | 2024-09-23T08:44:56Z |
| format | Article |
| id | mit-1721.1/147877 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:44:56Z |
| publishDate | 2023 |
| publisher | eLife Sciences Publications, Ltd |
| record_format | dspace |
| spelling | mit-1721.1/1478772023-02-04T03:46:26Z Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis Miettinen, Teemu P Ly, Kevin S Lam, Alice Manalis, Scott R Massachusetts Institute of Technology. Department of Biological Engineering <jats:p>Cell mass and composition change with cell cycle progression. Our previous work characterized buoyant mass dynamics in mitosis (Miettinen et al., 2019), but how dry mass and cell composition change in mitosis has remained unclear. To better understand mitotic cell growth and compositional changes, we develop a single-cell approach for monitoring dry mass and the density of that dry mass every ~75 s with 1.3% and 0.3% measurement precision, respectively. We find that suspension grown mammalian cells lose dry mass and increase dry mass density following mitotic entry. These changes display large, non-genetic cell-to-cell variability, and the changes are reversed at metaphase-anaphase transition, after which dry mass continues accumulating. The change in dry mass density causes buoyant and dry mass to differ specifically in early mitosis, thus reconciling existing literature on mitotic cell growth. Mechanistically, cells in early mitosis increase lysosomal exocytosis, and inhibition of lysosomal exocytosis decreases the dry mass loss and dry mass density increase in mitosis. Overall, our work provides a new approach for monitoring single-cell dry mass and dry mass density, and reveals that mitosis is coupled to extensive exocytosis-mediated secretion of cellular contents.</jats:p> 2023-02-03T19:46:58Z 2023-02-03T19:46:58Z 2022 2023-02-03T19:41:03Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147877 Miettinen, Teemu P, Ly, Kevin S, Lam, Alice and Manalis, Scott R. 2022. "Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis." eLife, 11. en 10.7554/ELIFE.76664 eLife Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf eLife Sciences Publications, Ltd eLife |
| spellingShingle | Miettinen, Teemu P Ly, Kevin S Lam, Alice Manalis, Scott R Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis |
| title | Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis |
| title_full | Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis |
| title_fullStr | Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis |
| title_full_unstemmed | Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis |
| title_short | Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis |
| title_sort | single cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis |
| url | https://hdl.handle.net/1721.1/147877 |
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