Genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend Panamanian golden frogs from disease
We designed two probiotic treatments to control chytridiomycosis caused by Batrachochytrium dendrobatidis (Bd) on infected Panamanian golden frogs (Atelopus zeteki), a species that is thought to be extinct in the wild due to Bd. The first approach disrupted the existing skin microbe community with a...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2023
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| Online Access: | https://hdl.handle.net/1721.1/147931 |
| _version_ | 1826218078614061056 |
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| author | Becker, Matthew H Brophy, Jennifer AN Barrett, Kevin Bronikowski, Ed Evans, Matthew Glassey, Emerson Kaganer, Alyssa W Klocke, Blake Lassiter, Elliot Meyer, Adam J Muletz-Wolz, Carly R Fleischer, Robert C Voigt, Christopher A Gratwicke, Brian |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Becker, Matthew H Brophy, Jennifer AN Barrett, Kevin Bronikowski, Ed Evans, Matthew Glassey, Emerson Kaganer, Alyssa W Klocke, Blake Lassiter, Elliot Meyer, Adam J Muletz-Wolz, Carly R Fleischer, Robert C Voigt, Christopher A Gratwicke, Brian |
| author_sort | Becker, Matthew H |
| collection | MIT |
| description | We designed two probiotic treatments to control chytridiomycosis caused by Batrachochytrium dendrobatidis (Bd) on infected Panamanian golden frogs (Atelopus zeteki), a species that is thought to be extinct in the wild due to Bd. The first approach disrupted the existing skin microbe community with antibiotics then exposed the frogs to a core golden frog skin microbe (Diaphorobacter sp.) that we genetically modified to produce high titers of violacein, a known antifungal compound. One day following probiotic treatment, the engineered Diaphorobacter and the violacein-producing pathway could be detected on the frogs but the treatment failed to improve frog survival when exposed to Bd. The second approach exposed frogs to the genetically modified bacterium mixed into a consortium with six other known anti-Bd bacteria isolated from captive A. zeteki, with no preliminary antibiotic treatment. The consortium treatment increased the frequency and abundance of three probiotic isolates (Janthinobacterium, Chryseobacterium, and Stenotrophomonas) and these persisted on the skin 4 weeks after probiotic treatment. There was a temporary increase in the frequency and abundance of three other probiotics isolates (Masillia, Serratia, and Pseudomonas) and the engineered Diaphorobacter isolate, but they subsequently disappeared from the skin. This treatment also failed to reduce frog mortality upon exposure. |
| first_indexed | 2024-09-23T17:13:28Z |
| format | Article |
| id | mit-1721.1/147931 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T17:13:28Z |
| publishDate | 2023 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1479312023-02-08T03:00:50Z Genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend Panamanian golden frogs from disease Becker, Matthew H Brophy, Jennifer AN Barrett, Kevin Bronikowski, Ed Evans, Matthew Glassey, Emerson Kaganer, Alyssa W Klocke, Blake Lassiter, Elliot Meyer, Adam J Muletz-Wolz, Carly R Fleischer, Robert C Voigt, Christopher A Gratwicke, Brian Massachusetts Institute of Technology. Department of Biological Engineering We designed two probiotic treatments to control chytridiomycosis caused by Batrachochytrium dendrobatidis (Bd) on infected Panamanian golden frogs (Atelopus zeteki), a species that is thought to be extinct in the wild due to Bd. The first approach disrupted the existing skin microbe community with antibiotics then exposed the frogs to a core golden frog skin microbe (Diaphorobacter sp.) that we genetically modified to produce high titers of violacein, a known antifungal compound. One day following probiotic treatment, the engineered Diaphorobacter and the violacein-producing pathway could be detected on the frogs but the treatment failed to improve frog survival when exposed to Bd. The second approach exposed frogs to the genetically modified bacterium mixed into a consortium with six other known anti-Bd bacteria isolated from captive A. zeteki, with no preliminary antibiotic treatment. The consortium treatment increased the frequency and abundance of three probiotic isolates (Janthinobacterium, Chryseobacterium, and Stenotrophomonas) and these persisted on the skin 4 weeks after probiotic treatment. There was a temporary increase in the frequency and abundance of three other probiotics isolates (Masillia, Serratia, and Pseudomonas) and the engineered Diaphorobacter isolate, but they subsequently disappeared from the skin. This treatment also failed to reduce frog mortality upon exposure. 2023-02-07T16:48:27Z 2023-02-07T16:48:27Z 2021 2023-02-07T16:37:51Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147931 Becker, Matthew H, Brophy, Jennifer AN, Barrett, Kevin, Bronikowski, Ed, Evans, Matthew et al. 2021. "Genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend Panamanian golden frogs from disease." ISME Communications, 1 (1). en 10.1038/S43705-021-00044-W ISME Communications Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Nature |
| spellingShingle | Becker, Matthew H Brophy, Jennifer AN Barrett, Kevin Bronikowski, Ed Evans, Matthew Glassey, Emerson Kaganer, Alyssa W Klocke, Blake Lassiter, Elliot Meyer, Adam J Muletz-Wolz, Carly R Fleischer, Robert C Voigt, Christopher A Gratwicke, Brian Genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend Panamanian golden frogs from disease |
| title | Genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend Panamanian golden frogs from disease |
| title_full | Genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend Panamanian golden frogs from disease |
| title_fullStr | Genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend Panamanian golden frogs from disease |
| title_full_unstemmed | Genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend Panamanian golden frogs from disease |
| title_short | Genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend Panamanian golden frogs from disease |
| title_sort | genetically modifying skin microbe to produce violacein and augmenting microbiome did not defend panamanian golden frogs from disease |
| url | https://hdl.handle.net/1721.1/147931 |
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