Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates
Living materials combine a material scaffold, that is often porous, with engineered cells that perform sensing, computing, and biosynthetic tasks. Designing such systems is difficult because little is known regarding signaling transport parameters in the material. Here, the development of a porous m...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
EMBO
2023
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| Online Access: | https://hdl.handle.net/1721.1/147939 |
| _version_ | 1826188764364406784 |
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| author | Vaiana, Christopher A Kim, Hyungseok Cottet, Jonathan Oai, Keiko Ge, Zhifei Conforti, Kameron King, Andrew M Meyer, Adam J Chen, Haorong Voigt, Christopher A Buie, Cullen R |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Vaiana, Christopher A Kim, Hyungseok Cottet, Jonathan Oai, Keiko Ge, Zhifei Conforti, Kameron King, Andrew M Meyer, Adam J Chen, Haorong Voigt, Christopher A Buie, Cullen R |
| author_sort | Vaiana, Christopher A |
| collection | MIT |
| description | Living materials combine a material scaffold, that is often porous, with engineered cells that perform sensing, computing, and biosynthetic tasks. Designing such systems is difficult because little is known regarding signaling transport parameters in the material. Here, the development of a porous microplate is presented. Hydrogel barriers between wells have a porosity of 60% and a tortuosity factor of 1.6, allowing molecular diffusion between wells. The permeability of dyes, antibiotics, inducers, and quorum signals between wells were characterized. A "sentinel" strain was constructed by introducing orthogonal sensors into the genome of Escherichia coli MG1655 for IPTG, anhydrotetracycline, L-arabinose, and four quorum signals. The strain's response to inducer diffusion through the wells was quantified up to 14 mm, and quorum and antibacterial signaling were measured over 16 h. Signaling distance is dictated by hydrogel adsorption, quantified using a linear finite element model that yields adsorption coefficients from 0 to 0.1 mol m-3 . Parameters derived herein will aid the design of living materials for pathogen remediation, computation, and self-organizing biofilms. |
| first_indexed | 2024-09-23T08:04:23Z |
| format | Article |
| id | mit-1721.1/147939 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:04:23Z |
| publishDate | 2023 |
| publisher | EMBO |
| record_format | dspace |
| spelling | mit-1721.1/1479392023-02-08T03:51:00Z Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates Vaiana, Christopher A Kim, Hyungseok Cottet, Jonathan Oai, Keiko Ge, Zhifei Conforti, Kameron King, Andrew M Meyer, Adam J Chen, Haorong Voigt, Christopher A Buie, Cullen R Massachusetts Institute of Technology. Department of Biological Engineering Living materials combine a material scaffold, that is often porous, with engineered cells that perform sensing, computing, and biosynthetic tasks. Designing such systems is difficult because little is known regarding signaling transport parameters in the material. Here, the development of a porous microplate is presented. Hydrogel barriers between wells have a porosity of 60% and a tortuosity factor of 1.6, allowing molecular diffusion between wells. The permeability of dyes, antibiotics, inducers, and quorum signals between wells were characterized. A "sentinel" strain was constructed by introducing orthogonal sensors into the genome of Escherichia coli MG1655 for IPTG, anhydrotetracycline, L-arabinose, and four quorum signals. The strain's response to inducer diffusion through the wells was quantified up to 14 mm, and quorum and antibacterial signaling were measured over 16 h. Signaling distance is dictated by hydrogel adsorption, quantified using a linear finite element model that yields adsorption coefficients from 0 to 0.1 mol m-3 . Parameters derived herein will aid the design of living materials for pathogen remediation, computation, and self-organizing biofilms. 2023-02-07T18:16:49Z 2023-02-07T18:16:49Z 2022 2023-02-07T17:47:45Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147939 Vaiana, Christopher A, Kim, Hyungseok, Cottet, Jonathan, Oai, Keiko, Ge, Zhifei et al. 2022. "Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates." Molecular Systems Biology, 18 (3). en 10.15252/MSB.202110785 Molecular Systems Biology Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf EMBO EMBO Press |
| spellingShingle | Vaiana, Christopher A Kim, Hyungseok Cottet, Jonathan Oai, Keiko Ge, Zhifei Conforti, Kameron King, Andrew M Meyer, Adam J Chen, Haorong Voigt, Christopher A Buie, Cullen R Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates |
| title | Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates |
| title_full | Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates |
| title_fullStr | Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates |
| title_full_unstemmed | Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates |
| title_short | Characterizing chemical signaling between engineered “microbial sentinels” in porous microplates |
| title_sort | characterizing chemical signaling between engineered microbial sentinels in porous microplates |
| url | https://hdl.handle.net/1721.1/147939 |
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