Principles for the design of multicellular engineered living systems
<jats:p> Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engineered living systems (M-CELS)...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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AIP Publishing
2023
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| Online Access: | https://hdl.handle.net/1721.1/147943 |
| _version_ | 1826196727291445248 |
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| author | Aydin, Onur Passaro, Austin P Raman, Ritu Spellicy, Samantha E Weinberg, Robert P Kamm, Roger D Sample, Matthew Truskey, George A Zartman, Jeremiah Dar, Roy D Palacios, Sebastian Wang, Jason Tordoff, Jesse Montserrat, Nuria Bashir, Rashid Saif, M Taher A Weiss, Ron |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Aydin, Onur Passaro, Austin P Raman, Ritu Spellicy, Samantha E Weinberg, Robert P Kamm, Roger D Sample, Matthew Truskey, George A Zartman, Jeremiah Dar, Roy D Palacios, Sebastian Wang, Jason Tordoff, Jesse Montserrat, Nuria Bashir, Rashid Saif, M Taher A Weiss, Ron |
| author_sort | Aydin, Onur |
| collection | MIT |
| description | <jats:p> Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engineered living systems (M-CELS) from biological parts, forming functional modules integrated into living machines. These cognizant design principles for living systems encompass novel genetic circuit manipulation, self-assembly, cell–cell/matrix communication, and artificial tissues/organs enabled through systems biology, bioinformatics, computational biology, genetic engineering, and microfluidics. Here, we introduce design principles and a blueprint for forward production of robust and standardized M-CELS, which may undergo variable reiterations through the classic design-build-test-debug cycle. This Review provides practical and theoretical frameworks to forward-design, control, and optimize novel M-CELS. Potential applications include biopharmaceuticals, bioreactor factories, biofuels, environmental bioremediation, cellular computing, biohybrid digital technology, and experimental investigations into mechanisms of multicellular organisms normally hidden inside the “black box” of living cells. </jats:p> |
| first_indexed | 2024-09-23T10:37:02Z |
| format | Article |
| id | mit-1721.1/147943 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:37:02Z |
| publishDate | 2023 |
| publisher | AIP Publishing |
| record_format | dspace |
| spelling | mit-1721.1/1479432023-02-08T03:35:40Z Principles for the design of multicellular engineered living systems Aydin, Onur Passaro, Austin P Raman, Ritu Spellicy, Samantha E Weinberg, Robert P Kamm, Roger D Sample, Matthew Truskey, George A Zartman, Jeremiah Dar, Roy D Palacios, Sebastian Wang, Jason Tordoff, Jesse Montserrat, Nuria Bashir, Rashid Saif, M Taher A Weiss, Ron Massachusetts Institute of Technology. Department of Biological Engineering <jats:p> Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engineered living systems (M-CELS) from biological parts, forming functional modules integrated into living machines. These cognizant design principles for living systems encompass novel genetic circuit manipulation, self-assembly, cell–cell/matrix communication, and artificial tissues/organs enabled through systems biology, bioinformatics, computational biology, genetic engineering, and microfluidics. Here, we introduce design principles and a blueprint for forward production of robust and standardized M-CELS, which may undergo variable reiterations through the classic design-build-test-debug cycle. This Review provides practical and theoretical frameworks to forward-design, control, and optimize novel M-CELS. Potential applications include biopharmaceuticals, bioreactor factories, biofuels, environmental bioremediation, cellular computing, biohybrid digital technology, and experimental investigations into mechanisms of multicellular organisms normally hidden inside the “black box” of living cells. </jats:p> 2023-02-07T18:48:32Z 2023-02-07T18:48:32Z 2022 2023-02-07T18:33:59Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/147943 Aydin, Onur, Passaro, Austin P, Raman, Ritu, Spellicy, Samantha E, Weinberg, Robert P et al. 2022. "Principles for the design of multicellular engineered living systems." APL Bioengineering, 6 (1). en 10.1063/5.0076635 APL Bioengineering Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf AIP Publishing American Institute of Physics (AIP) |
| spellingShingle | Aydin, Onur Passaro, Austin P Raman, Ritu Spellicy, Samantha E Weinberg, Robert P Kamm, Roger D Sample, Matthew Truskey, George A Zartman, Jeremiah Dar, Roy D Palacios, Sebastian Wang, Jason Tordoff, Jesse Montserrat, Nuria Bashir, Rashid Saif, M Taher A Weiss, Ron Principles for the design of multicellular engineered living systems |
| title | Principles for the design of multicellular engineered living systems |
| title_full | Principles for the design of multicellular engineered living systems |
| title_fullStr | Principles for the design of multicellular engineered living systems |
| title_full_unstemmed | Principles for the design of multicellular engineered living systems |
| title_short | Principles for the design of multicellular engineered living systems |
| title_sort | principles for the design of multicellular engineered living systems |
| url | https://hdl.handle.net/1721.1/147943 |
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