7.344 Directed Evolution: Engineering Biocatalysts, Spring 2008
Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic pro...
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| Language: | en-US |
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2023
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| Online Access: | https://hdl.handle.net/1721.1/148328 |
| _version_ | 1811074699431510016 |
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| author | Love, Kerry |
| author_facet | Love, Kerry |
| author_sort | Love, Kerry |
| collection | MIT |
| description | Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. Many instructors of the Advanced Undergraduate Seminars are postdoctoral scientists with a strong interest in teaching. |
| first_indexed | 2024-09-23T09:53:57Z |
| id | mit-1721.1/148328 |
| institution | Massachusetts Institute of Technology |
| language | en-US |
| last_indexed | 2024-09-23T09:53:57Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | mit-1721.1/1483282023-03-07T04:20:45Z 7.344 Directed Evolution: Engineering Biocatalysts, Spring 2008 Directed Evolution: Engineering Biocatalysts Love, Kerry evolution biocatalyst mutation library recombination directed evolution enzyme point mutation mutagenesis DNA evolution gene complementation affinity phage ribosome display yeast surface display bacterial cell surface display IVC FACS active site 261004 Toxicology Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. Many instructors of the Advanced Undergraduate Seminars are postdoctoral scientists with a strong interest in teaching. 2023-03-06T16:54:13Z 2023-03-06T16:54:13Z 2008-06 2023-03-06T16:54:21Z 7.344-Spring2008 7.344 IMSCP-MD5-6321f8c1769ca121b1d6228e00b8a6f1 https://hdl.handle.net/1721.1/148328 en-US This site (c) Massachusetts Institute of Technology 2023. Content within individual courses is (c) by the individual authors unless otherwise noted. The Massachusetts Institute of Technology is providing this Work (as defined below) under the terms of this Creative Commons public license ("CCPL" or "license") unless otherwise noted. The Work is protected by copyright and/or other applicable law. Any use of the work other than as authorized under this license is prohibited. By exercising any of the rights to the Work provided here, You (as defined below) accept and agree to be bound by the terms of this license. The Licensor, the Massachusetts Institute of Technology, grants You the rights contained here in consideration of Your acceptance of such terms and conditions. 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| spellingShingle | evolution biocatalyst mutation library recombination directed evolution enzyme point mutation mutagenesis DNA evolution gene complementation affinity phage ribosome display yeast surface display bacterial cell surface display IVC FACS active site 261004 Toxicology Love, Kerry 7.344 Directed Evolution: Engineering Biocatalysts, Spring 2008 |
| title | 7.344 Directed Evolution: Engineering Biocatalysts, Spring 2008 |
| title_full | 7.344 Directed Evolution: Engineering Biocatalysts, Spring 2008 |
| title_fullStr | 7.344 Directed Evolution: Engineering Biocatalysts, Spring 2008 |
| title_full_unstemmed | 7.344 Directed Evolution: Engineering Biocatalysts, Spring 2008 |
| title_short | 7.344 Directed Evolution: Engineering Biocatalysts, Spring 2008 |
| title_sort | 7 344 directed evolution engineering biocatalysts spring 2008 |
| topic | evolution biocatalyst mutation library recombination directed evolution enzyme point mutation mutagenesis DNA evolution gene complementation affinity phage ribosome display yeast surface display bacterial cell surface display IVC FACS active site 261004 Toxicology |
| url | https://hdl.handle.net/1721.1/148328 |
| work_keys_str_mv | AT lovekerry 7344directedevolutionengineeringbiocatalystsspring2008 AT lovekerry directedevolutionengineeringbiocatalysts |