Megakaryocytes respond during sepsis and display innate immune cell behaviors
Megakaryocytes (MKs) are precursors to platelets, the second most abundant cells in the peripheral circulation. However, while platelets are known to participate in immune responses and play significant functions during infections, the role of MKs within the immune system remains largely unexplored....
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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Frontiers Media SA
2023
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| Online Access: | https://hdl.handle.net/1721.1/148459 |
| _version_ | 1811092380448718848 |
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| author | Frydman, Galit H. Ellett, Felix Jorgensen, Julianne Marand, Anika L. Zukerberg, Lawrence Selig, Martin K. Tessier, Shannon N. Wong, Keith H. K. Olaleye, David Vanderburg, Charles R. Fox, James G. Tompkins, Ronald G. Irimia, Daniel |
| author2 | Massachusetts Institute of Technology. Division of Comparative Medicine |
| author_facet | Massachusetts Institute of Technology. Division of Comparative Medicine Frydman, Galit H. Ellett, Felix Jorgensen, Julianne Marand, Anika L. Zukerberg, Lawrence Selig, Martin K. Tessier, Shannon N. Wong, Keith H. K. Olaleye, David Vanderburg, Charles R. Fox, James G. Tompkins, Ronald G. Irimia, Daniel |
| author_sort | Frydman, Galit H. |
| collection | MIT |
| description | Megakaryocytes (MKs) are precursors to platelets, the second most abundant cells in the peripheral circulation. However, while platelets are known to participate in immune responses and play significant functions during infections, the role of MKs within the immune system remains largely unexplored. Histological studies of sepsis patients identified increased nucleated CD61+ cells (MKs) in the lungs, and CD61+ staining (likely platelets within microthrombi) in the kidneys, which correlated with the development of organ dysfunction. Detailed imaging cytometry of peripheral blood from patients with sepsis found significantly higher MK counts, which we predict would likely be misclassified by automated hematology analyzers as leukocytes. Utilizing in vitro techniques, we show that both stem cell derived MKs (SC MKs) and cells from the human megakaryoblastic leukemia cell line, Meg-01, undergo chemotaxis, interact with bacteria, and are capable of releasing chromatin webs in response to various pathogenic stimuli. Together, our observations suggest that MK cells display some basic innate immune cell behaviors and may actively respond and play functional roles in the pathophysiology of sepsis. |
| first_indexed | 2024-09-23T15:17:15Z |
| format | Article |
| id | mit-1721.1/148459 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T15:17:15Z |
| publishDate | 2023 |
| publisher | Frontiers Media SA |
| record_format | dspace |
| spelling | mit-1721.1/1484592024-01-23T18:54:04Z Megakaryocytes respond during sepsis and display innate immune cell behaviors Frydman, Galit H. Ellett, Felix Jorgensen, Julianne Marand, Anika L. Zukerberg, Lawrence Selig, Martin K. Tessier, Shannon N. Wong, Keith H. K. Olaleye, David Vanderburg, Charles R. Fox, James G. Tompkins, Ronald G. Irimia, Daniel Massachusetts Institute of Technology. Division of Comparative Medicine Massachusetts Institute of Technology. Department of Biological Engineering Immunology Immunology and Allergy Megakaryocytes (MKs) are precursors to platelets, the second most abundant cells in the peripheral circulation. However, while platelets are known to participate in immune responses and play significant functions during infections, the role of MKs within the immune system remains largely unexplored. Histological studies of sepsis patients identified increased nucleated CD61+ cells (MKs) in the lungs, and CD61+ staining (likely platelets within microthrombi) in the kidneys, which correlated with the development of organ dysfunction. Detailed imaging cytometry of peripheral blood from patients with sepsis found significantly higher MK counts, which we predict would likely be misclassified by automated hematology analyzers as leukocytes. Utilizing in vitro techniques, we show that both stem cell derived MKs (SC MKs) and cells from the human megakaryoblastic leukemia cell line, Meg-01, undergo chemotaxis, interact with bacteria, and are capable of releasing chromatin webs in response to various pathogenic stimuli. Together, our observations suggest that MK cells display some basic innate immune cell behaviors and may actively respond and play functional roles in the pathophysiology of sepsis. 2023-03-10T17:11:15Z 2023-03-10T17:11:15Z 2023-03-02 Article http://purl.org/eprint/type/JournalArticle 1664-3224 https://hdl.handle.net/1721.1/148459 Frydman, Galit H., Ellett, Felix, Jorgensen, Julianne, Marand, Anika L., Zukerberg, Lawrence et al. 2023. "Megakaryocytes respond during sepsis and display innate immune cell behaviors." 14. 10.3389/fimmu.2023.1083339 Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Frontiers Media SA Frontiers |
| spellingShingle | Immunology Immunology and Allergy Frydman, Galit H. Ellett, Felix Jorgensen, Julianne Marand, Anika L. Zukerberg, Lawrence Selig, Martin K. Tessier, Shannon N. Wong, Keith H. K. Olaleye, David Vanderburg, Charles R. Fox, James G. Tompkins, Ronald G. Irimia, Daniel Megakaryocytes respond during sepsis and display innate immune cell behaviors |
| title | Megakaryocytes respond during sepsis and display innate immune cell behaviors |
| title_full | Megakaryocytes respond during sepsis and display innate immune cell behaviors |
| title_fullStr | Megakaryocytes respond during sepsis and display innate immune cell behaviors |
| title_full_unstemmed | Megakaryocytes respond during sepsis and display innate immune cell behaviors |
| title_short | Megakaryocytes respond during sepsis and display innate immune cell behaviors |
| title_sort | megakaryocytes respond during sepsis and display innate immune cell behaviors |
| topic | Immunology Immunology and Allergy |
| url | https://hdl.handle.net/1721.1/148459 |
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