The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment
Background: Atopic dermatitis (AD) is characterized by an altered skin microbiome dominantly colonized by S. aureus. Standard treatment includes emollients, anti-inflammatory medications and antiseptics. Objectives: To characterize changes in the skin microbiome during treatment for AD. Method...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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Frontiers Media SA
2023
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| Online Access: | https://hdl.handle.net/1721.1/148604 |
| _version_ | 1811088673606729728 |
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| author | Khadka, Veda D Key, Felix M Romo-González, Carolina Martínez-Gayosso, Adrián Campos-Cabrera, Blanca L Gerónimo-Gallegos, Armando Lynn, Tucker C Durán-McKinster, Carola Coria-Jiménez, Rafael Lieberman, Tami D García-Romero, Maria T |
| author2 | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Khadka, Veda D Key, Felix M Romo-González, Carolina Martínez-Gayosso, Adrián Campos-Cabrera, Blanca L Gerónimo-Gallegos, Armando Lynn, Tucker C Durán-McKinster, Carola Coria-Jiménez, Rafael Lieberman, Tami D García-Romero, Maria T |
| author_sort | Khadka, Veda D |
| collection | MIT |
| description | Background: Atopic dermatitis (AD) is characterized by an altered skin microbiome dominantly colonized by S. aureus. Standard treatment includes emollients, anti-inflammatory medications and antiseptics.
Objectives: To characterize changes in the skin microbiome during treatment for AD.
Methods: The skin microbiomes of children with moderate-to-severe AD and healthy children were investigated in a longitudinal prospective study. Patients with AD were randomized to receive either standard treatment with emollients and topical corticosteroids or standard treatment with the addition of dilute bleach baths (DBB) and sampled at four visits over a three-month period. At each visit, severity of AD was measured, swabs were taken from four body sites and the composition of the microbiome at those sites was assessed using 16S rRNA amplification.
Results: We included 14 healthy controls and 28 patients. We found high relative abundances of S. aureus in patients, which correlated with AD severity and reduced apparent alpha diversity. As disease severity improved with treatment, the abundance of S. aureus decreased, gradually becoming more similar to the microbiomes of healthy controls. After treatment, patients who received DBB had a significantly lower abundance of S. aureus than those who received only standard treatment.
Conclusions: There are clear differences in the skin microbiome of healthy controls and AD patients that diminish with treatment. After three months, the addition of DBB to standard treatment had significantly decreased the S. aureus burden, supporting its use as a therapeutic option. Further study in double-blinded trials is needed. |
| first_indexed | 2024-09-23T14:05:44Z |
| format | Article |
| id | mit-1721.1/148604 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T14:05:44Z |
| publishDate | 2023 |
| publisher | Frontiers Media SA |
| record_format | dspace |
| spelling | mit-1721.1/1486042023-03-18T03:18:59Z The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment Khadka, Veda D Key, Felix M Romo-González, Carolina Martínez-Gayosso, Adrián Campos-Cabrera, Blanca L Gerónimo-Gallegos, Armando Lynn, Tucker C Durán-McKinster, Carola Coria-Jiménez, Rafael Lieberman, Tami D García-Romero, Maria T Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Background: Atopic dermatitis (AD) is characterized by an altered skin microbiome dominantly colonized by S. aureus. Standard treatment includes emollients, anti-inflammatory medications and antiseptics. Objectives: To characterize changes in the skin microbiome during treatment for AD. Methods: The skin microbiomes of children with moderate-to-severe AD and healthy children were investigated in a longitudinal prospective study. Patients with AD were randomized to receive either standard treatment with emollients and topical corticosteroids or standard treatment with the addition of dilute bleach baths (DBB) and sampled at four visits over a three-month period. At each visit, severity of AD was measured, swabs were taken from four body sites and the composition of the microbiome at those sites was assessed using 16S rRNA amplification. Results: We included 14 healthy controls and 28 patients. We found high relative abundances of S. aureus in patients, which correlated with AD severity and reduced apparent alpha diversity. As disease severity improved with treatment, the abundance of S. aureus decreased, gradually becoming more similar to the microbiomes of healthy controls. After treatment, patients who received DBB had a significantly lower abundance of S. aureus than those who received only standard treatment. Conclusions: There are clear differences in the skin microbiome of healthy controls and AD patients that diminish with treatment. After three months, the addition of DBB to standard treatment had significantly decreased the S. aureus burden, supporting its use as a therapeutic option. Further study in double-blinded trials is needed. 2023-03-17T17:01:49Z 2023-03-17T17:01:49Z 2021 2023-03-17T16:57:47Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/148604 Khadka, Veda D, Key, Felix M, Romo-González, Carolina, Martínez-Gayosso, Adrián, Campos-Cabrera, Blanca L et al. 2021. "The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment." Frontiers in Cellular and Infection Microbiology, 11. en 10.3389/FCIMB.2021.720674 Frontiers in Cellular and Infection Microbiology Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/ application/pdf Frontiers Media SA Frontiers |
| spellingShingle | Khadka, Veda D Key, Felix M Romo-González, Carolina Martínez-Gayosso, Adrián Campos-Cabrera, Blanca L Gerónimo-Gallegos, Armando Lynn, Tucker C Durán-McKinster, Carola Coria-Jiménez, Rafael Lieberman, Tami D García-Romero, Maria T The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment |
| title | The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment |
| title_full | The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment |
| title_fullStr | The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment |
| title_full_unstemmed | The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment |
| title_short | The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment |
| title_sort | skin microbiome of patients with atopic dermatitis normalizes gradually during treatment |
| url | https://hdl.handle.net/1721.1/148604 |
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