Large-scale voltage imaging in behaving mice using targeted illumination
Recent improvements in genetically encoded voltage indicators enabled optical imaging of action potentials and subthreshold transmembrane voltage in vivo. To perform high-speed voltage imaging of many neurons simultaneously over a large anatomical area, widefield microscopy remains an essential tool...
| Main Authors: | , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2023
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| Online Access: | https://hdl.handle.net/1721.1/148692 |
| _version_ | 1826207178843750400 |
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| author | Xiao, Sheng Lowet, Eric Gritton, Howard J Fabris, Pierre Wang, Yangyang Sherman, Jack Mount, Rebecca A Tseng, Hua-an Man, Heng-Ye Straub, Christoph Piatkevich, Kiryl D Boyden, Edward S Mertz, Jerome Han, Xue |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Xiao, Sheng Lowet, Eric Gritton, Howard J Fabris, Pierre Wang, Yangyang Sherman, Jack Mount, Rebecca A Tseng, Hua-an Man, Heng-Ye Straub, Christoph Piatkevich, Kiryl D Boyden, Edward S Mertz, Jerome Han, Xue |
| author_sort | Xiao, Sheng |
| collection | MIT |
| description | Recent improvements in genetically encoded voltage indicators enabled optical imaging of action potentials and subthreshold transmembrane voltage in vivo. To perform high-speed voltage imaging of many neurons simultaneously over a large anatomical area, widefield microscopy remains an essential tool. However, the lack of optical sectioning makes widefield microscopy prone to background cross-contamination. We implemented a digital-micromirror-device-based targeted illumination strategy to restrict illumination to the cells of interest and quantified the resulting improvement both theoretically and experimentally with SomArchon expressing neurons. We found that targeted illumination increased SomArchon signal contrast, decreased photobleaching, and reduced background cross-contamination. With the use of a high-speed, large-area sCMOS camera, we routinely imaged tens of spiking neurons simultaneously over minutes in behaving mice. Thus, the targeted illumination strategy described here offers a simple solution for widefield voltage imaging of many neurons over a large field of view in behaving animals. |
| first_indexed | 2024-09-23T13:45:16Z |
| format | Article |
| id | mit-1721.1/148692 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:45:16Z |
| publishDate | 2023 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1486922023-03-25T03:22:50Z Large-scale voltage imaging in behaving mice using targeted illumination Xiao, Sheng Lowet, Eric Gritton, Howard J Fabris, Pierre Wang, Yangyang Sherman, Jack Mount, Rebecca A Tseng, Hua-an Man, Heng-Ye Straub, Christoph Piatkevich, Kiryl D Boyden, Edward S Mertz, Jerome Han, Xue Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Recent improvements in genetically encoded voltage indicators enabled optical imaging of action potentials and subthreshold transmembrane voltage in vivo. To perform high-speed voltage imaging of many neurons simultaneously over a large anatomical area, widefield microscopy remains an essential tool. However, the lack of optical sectioning makes widefield microscopy prone to background cross-contamination. We implemented a digital-micromirror-device-based targeted illumination strategy to restrict illumination to the cells of interest and quantified the resulting improvement both theoretically and experimentally with SomArchon expressing neurons. We found that targeted illumination increased SomArchon signal contrast, decreased photobleaching, and reduced background cross-contamination. With the use of a high-speed, large-area sCMOS camera, we routinely imaged tens of spiking neurons simultaneously over minutes in behaving mice. Thus, the targeted illumination strategy described here offers a simple solution for widefield voltage imaging of many neurons over a large field of view in behaving animals. 2023-03-24T12:26:39Z 2023-03-24T12:26:39Z 2021 2023-03-24T12:09:55Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/148692 Xiao, Sheng, Lowet, Eric, Gritton, Howard J, Fabris, Pierre, Wang, Yangyang et al. 2021. "Large-scale voltage imaging in behaving mice using targeted illumination." iScience, 24 (11). en 10.1016/J.ISCI.2021.103263 iScience Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV Elsevier |
| spellingShingle | Xiao, Sheng Lowet, Eric Gritton, Howard J Fabris, Pierre Wang, Yangyang Sherman, Jack Mount, Rebecca A Tseng, Hua-an Man, Heng-Ye Straub, Christoph Piatkevich, Kiryl D Boyden, Edward S Mertz, Jerome Han, Xue Large-scale voltage imaging in behaving mice using targeted illumination |
| title | Large-scale voltage imaging in behaving mice using targeted illumination |
| title_full | Large-scale voltage imaging in behaving mice using targeted illumination |
| title_fullStr | Large-scale voltage imaging in behaving mice using targeted illumination |
| title_full_unstemmed | Large-scale voltage imaging in behaving mice using targeted illumination |
| title_short | Large-scale voltage imaging in behaving mice using targeted illumination |
| title_sort | large scale voltage imaging in behaving mice using targeted illumination |
| url | https://hdl.handle.net/1721.1/148692 |
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