An optical design enabling lightweight and large field-of-view head-mounted microscopes
Here we present a fluorescence microscope light path that enables imaging, during free behavior, of thousands of neurons in mice and hundreds of neurons in juvenile songbirds. The light path eliminates traditional illumination optics, allowing for head-mounted microscopes that have both a lower weig...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2023
|
| Online Access: | https://hdl.handle.net/1721.1/148776 |
| _version_ | 1826207051665113088 |
|---|---|
| author | Scherrer, Joseph R Lynch, Galen F Zhang, Jie J Fee, Michale S |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Scherrer, Joseph R Lynch, Galen F Zhang, Jie J Fee, Michale S |
| author_sort | Scherrer, Joseph R |
| collection | MIT |
| description | Here we present a fluorescence microscope light path that enables imaging, during free behavior, of thousands of neurons in mice and hundreds of neurons in juvenile songbirds. The light path eliminates traditional illumination optics, allowing for head-mounted microscopes that have both a lower weight and a larger field of view (FOV) than previously possible. Using this light path, we designed two microscopes: one optimized for FOV (~4 mm FOV; 1.4 g), and the other optimized for weight (1.0 mm FOV; 1.0 g). |
| first_indexed | 2024-09-23T13:42:45Z |
| format | Article |
| id | mit-1721.1/148776 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:42:45Z |
| publishDate | 2023 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1487762023-03-28T03:22:22Z An optical design enabling lightweight and large field-of-view head-mounted microscopes Scherrer, Joseph R Lynch, Galen F Zhang, Jie J Fee, Michale S Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Here we present a fluorescence microscope light path that enables imaging, during free behavior, of thousands of neurons in mice and hundreds of neurons in juvenile songbirds. The light path eliminates traditional illumination optics, allowing for head-mounted microscopes that have both a lower weight and a larger field of view (FOV) than previously possible. Using this light path, we designed two microscopes: one optimized for FOV (~4 mm FOV; 1.4 g), and the other optimized for weight (1.0 mm FOV; 1.0 g). 2023-03-27T13:55:24Z 2023-03-27T13:55:24Z 2023-03-16 2023-03-27T13:45:55Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/148776 Scherrer, Joseph R, Lynch, Galen F, Zhang, Jie J and Fee, Michale S. 2023. "An optical design enabling lightweight and large field-of-view head-mounted microscopes." Nature Methods. en 10.1038/s41592-023-01806-1 Nature Methods Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer Science and Business Media LLC bioRxiv |
| spellingShingle | Scherrer, Joseph R Lynch, Galen F Zhang, Jie J Fee, Michale S An optical design enabling lightweight and large field-of-view head-mounted microscopes |
| title | An optical design enabling lightweight and large field-of-view head-mounted microscopes |
| title_full | An optical design enabling lightweight and large field-of-view head-mounted microscopes |
| title_fullStr | An optical design enabling lightweight and large field-of-view head-mounted microscopes |
| title_full_unstemmed | An optical design enabling lightweight and large field-of-view head-mounted microscopes |
| title_short | An optical design enabling lightweight and large field-of-view head-mounted microscopes |
| title_sort | optical design enabling lightweight and large field of view head mounted microscopes |
| url | https://hdl.handle.net/1721.1/148776 |
| work_keys_str_mv | AT scherrerjosephr anopticaldesignenablinglightweightandlargefieldofviewheadmountedmicroscopes AT lynchgalenf anopticaldesignenablinglightweightandlargefieldofviewheadmountedmicroscopes AT zhangjiej anopticaldesignenablinglightweightandlargefieldofviewheadmountedmicroscopes AT feemichales anopticaldesignenablinglightweightandlargefieldofviewheadmountedmicroscopes AT scherrerjosephr opticaldesignenablinglightweightandlargefieldofviewheadmountedmicroscopes AT lynchgalenf opticaldesignenablinglightweightandlargefieldofviewheadmountedmicroscopes AT zhangjiej opticaldesignenablinglightweightandlargefieldofviewheadmountedmicroscopes AT feemichales opticaldesignenablinglightweightandlargefieldofviewheadmountedmicroscopes |