ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy
Studies of C. elegans will benefit from a powerful method for super-resolution imaging of proteins and mRNAs at any 3-D locations throughout the entire animal. Conventional methods of super-resolution imaging in C. elegans, such as STORM, PALM, SR-SIM and STED, are limited by imaging depths that are...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer US
2023
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| Online Access: | https://hdl.handle.net/1721.1/150771 |
| _version_ | 1811095412934705152 |
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| author | Yu, Chih-Chieh Orozco Cosio, Danielle M Boyden, Edward S |
| author_facet | Yu, Chih-Chieh Orozco Cosio, Danielle M Boyden, Edward S |
| author_sort | Yu, Chih-Chieh |
| collection | MIT |
| description | Studies of C. elegans will benefit from a powerful method for super-resolution imaging of proteins and mRNAs at any 3-D locations throughout the entire animal. Conventional methods of super-resolution imaging in C. elegans, such as STORM, PALM, SR-SIM and STED, are limited by imaging depths that are insufficient to map the entire depth of adult worms, and involve hardware that may not be accessible to all labs. We recently developed expansion of C. elegans (ExCel), a method for physically magnifying fixed whole animals of C. elegans with high isotropy, which provides effective resolutions finer than the diffraction limit, across the entire animal, on conventional confocal microscopes. In this chapter, we present a family of three detailed ExCel protocols. The standard ExCel protocol features simultaneous readout of diverse molecules (fluorescent proteins, RNA, DNA, and general anatomy), all at ~70 nm resolution (~3.5× linear expansion). The epitope-preserving ExCel protocol enables imaging of endogenous proteins with off-the-shelf antibodies, at a ~ 100 nm resolution (~2.8× linear expansion). The iterative ExCel protocol allows readout of fluorescent proteins at ~25 nm resolution (~20× linear expansion). The protocols described here comprise a versatile toolbox for super-resolution imaging of C. elegans. |
| first_indexed | 2024-09-23T16:16:21Z |
| format | Article |
| id | mit-1721.1/150771 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T16:16:21Z |
| publishDate | 2023 |
| publisher | Springer US |
| record_format | dspace |
| spelling | mit-1721.1/1507712023-05-19T03:02:50Z ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy Yu, Chih-Chieh Orozco Cosio, Danielle M Boyden, Edward S Studies of C. elegans will benefit from a powerful method for super-resolution imaging of proteins and mRNAs at any 3-D locations throughout the entire animal. Conventional methods of super-resolution imaging in C. elegans, such as STORM, PALM, SR-SIM and STED, are limited by imaging depths that are insufficient to map the entire depth of adult worms, and involve hardware that may not be accessible to all labs. We recently developed expansion of C. elegans (ExCel), a method for physically magnifying fixed whole animals of C. elegans with high isotropy, which provides effective resolutions finer than the diffraction limit, across the entire animal, on conventional confocal microscopes. In this chapter, we present a family of three detailed ExCel protocols. The standard ExCel protocol features simultaneous readout of diverse molecules (fluorescent proteins, RNA, DNA, and general anatomy), all at ~70 nm resolution (~3.5× linear expansion). The epitope-preserving ExCel protocol enables imaging of endogenous proteins with off-the-shelf antibodies, at a ~ 100 nm resolution (~2.8× linear expansion). The iterative ExCel protocol allows readout of fluorescent proteins at ~25 nm resolution (~20× linear expansion). The protocols described here comprise a versatile toolbox for super-resolution imaging of C. elegans. 2023-05-18T13:34:59Z 2023-05-18T13:34:59Z 2022 2023-05-18T13:31:00Z Article http://purl.org/eprint/type/BookItem https://hdl.handle.net/1721.1/150771 Yu, Chih-Chieh, Orozco Cosio, Danielle M and Boyden, Edward S. 2022. "ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy." Methods Mol Biol, 2468. en 10.1007/978-1-0716-2181-3_9 Methods Mol Biol Creative Commons Attribution-Noncommercial-Share Alike https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer US MIT web |
| spellingShingle | Yu, Chih-Chieh Orozco Cosio, Danielle M Boyden, Edward S ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy |
| title | ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy |
| title_full | ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy |
| title_fullStr | ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy |
| title_full_unstemmed | ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy |
| title_short | ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy |
| title_sort | excel super resolution imaging of c elegans with expansion microscopy |
| url | https://hdl.handle.net/1721.1/150771 |
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