DNA nanopores as artificial membrane channels for bioprotonics
<jats:title>Abstract</jats:title><jats:p>Biological membrane channels mediate information exchange between cells and facilitate molecular recognition. While tuning the shape and function of membrane channels for precision molecular sensing via de-novo routes is complex, an even mor...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2023
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| Online Access: | https://hdl.handle.net/1721.1/152514 |
| _version_ | 1826197784899878912 |
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| author | Luo, Le Manda, Swathi Park, Yunjeong Demir, Busra Sanchez, Jesse Anantram, M. P. Oren, Ersin Emre Gopinath, Ashwin Rolandi, Marco |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Luo, Le Manda, Swathi Park, Yunjeong Demir, Busra Sanchez, Jesse Anantram, M. P. Oren, Ersin Emre Gopinath, Ashwin Rolandi, Marco |
| author_sort | Luo, Le |
| collection | MIT |
| description | <jats:title>Abstract</jats:title><jats:p>Biological membrane channels mediate information exchange between cells and facilitate molecular recognition. While tuning the shape and function of membrane channels for precision molecular sensing via de-novo routes is complex, an even more significant challenge is interfacing membrane channels with electronic devices for signal readout, which results in low efficiency of information transfer - one of the major barriers to the continued development of high-performance bioelectronic devices. To this end, we integrate membrane spanning DNA nanopores with bioprotonic contacts to create programmable, modular, and efficient artificial ion-channel interfaces. Here we show that cholesterol modified DNA nanopores spontaneously and with remarkable affinity span the lipid bilayer formed over the planar bio-protonic electrode surface and mediate proton transport across the bilayer. Using the ability to easily modify DNA nanostructures, we illustrate that this bioprotonic device can be programmed for electronic recognition of biomolecular signals such as presence of Streptavidin and the cardiac biomarker B-type natriuretic peptide, without modifying the biomolecules. We anticipate this robust interface will allow facile electronic measurement and quantification of biomolecules in a multiplexed manner.</jats:p> |
| first_indexed | 2024-09-23T10:53:06Z |
| format | Article |
| id | mit-1721.1/152514 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T10:53:06Z |
| publishDate | 2023 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1525142024-01-22T21:52:44Z DNA nanopores as artificial membrane channels for bioprotonics Luo, Le Manda, Swathi Park, Yunjeong Demir, Busra Sanchez, Jesse Anantram, M. P. Oren, Ersin Emre Gopinath, Ashwin Rolandi, Marco Massachusetts Institute of Technology. Department of Mechanical Engineering General Physics and Astronomy General Biochemistry, Genetics and Molecular Biology General Chemistry Multidisciplinary <jats:title>Abstract</jats:title><jats:p>Biological membrane channels mediate information exchange between cells and facilitate molecular recognition. While tuning the shape and function of membrane channels for precision molecular sensing via de-novo routes is complex, an even more significant challenge is interfacing membrane channels with electronic devices for signal readout, which results in low efficiency of information transfer - one of the major barriers to the continued development of high-performance bioelectronic devices. To this end, we integrate membrane spanning DNA nanopores with bioprotonic contacts to create programmable, modular, and efficient artificial ion-channel interfaces. Here we show that cholesterol modified DNA nanopores spontaneously and with remarkable affinity span the lipid bilayer formed over the planar bio-protonic electrode surface and mediate proton transport across the bilayer. Using the ability to easily modify DNA nanostructures, we illustrate that this bioprotonic device can be programmed for electronic recognition of biomolecular signals such as presence of Streptavidin and the cardiac biomarker B-type natriuretic peptide, without modifying the biomolecules. We anticipate this robust interface will allow facile electronic measurement and quantification of biomolecules in a multiplexed manner.</jats:p> 2023-10-20T18:58:04Z 2023-10-20T18:58:04Z 2023-09-04 2023-10-20T18:52:24Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/152514 Luo, Le, Manda, Swathi, Park, Yunjeong, Demir, Busra, Sanchez, Jesse et al. 2023. "DNA nanopores as artificial membrane channels for bioprotonics." Nature Communications, 14 (1). en 10.1038/s41467-023-40870-1 Nature Communications Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Springer Science and Business Media LLC |
| spellingShingle | General Physics and Astronomy General Biochemistry, Genetics and Molecular Biology General Chemistry Multidisciplinary Luo, Le Manda, Swathi Park, Yunjeong Demir, Busra Sanchez, Jesse Anantram, M. P. Oren, Ersin Emre Gopinath, Ashwin Rolandi, Marco DNA nanopores as artificial membrane channels for bioprotonics |
| title | DNA nanopores as artificial membrane channels for bioprotonics |
| title_full | DNA nanopores as artificial membrane channels for bioprotonics |
| title_fullStr | DNA nanopores as artificial membrane channels for bioprotonics |
| title_full_unstemmed | DNA nanopores as artificial membrane channels for bioprotonics |
| title_short | DNA nanopores as artificial membrane channels for bioprotonics |
| title_sort | dna nanopores as artificial membrane channels for bioprotonics |
| topic | General Physics and Astronomy General Biochemistry, Genetics and Molecular Biology General Chemistry Multidisciplinary |
| url | https://hdl.handle.net/1721.1/152514 |
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