A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens

A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens

Abstract Mapping genetic interactions is essential for determining gene function and defining novel biological pathways. We report a simple to use CRISPR interference (CRISPRi) based platform, compatible with Fluorescence Activated Cell Sorting (FACS)-based reporter screens, to query...

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Bibliographic Details
Main Authors: Guna, Alina, Page, Katharine R., Replogle, Joseph M., Esantsi, Theodore K., Wang, Maxine L., Weissman, Jonathan S., Voorhees, Rebecca M.
Other Authors: Whitehead Institute for Biomedical Research
Format: Article
Language:English
Published: BioMed Central 2023
Online Access:https://hdl.handle.net/1721.1/152913

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