Texaphyrin-Based Calcium Sensor for Multimodal Imaging
The ability to monitor intracellular calcium concentrations using fluorescent probes has led to important insights into biological signaling processes at the cellular level. An important challenge is to relate such measurements to broader patterns of signaling across fields of view that are inaccess...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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American Chemical Society (ACS)
2024
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| Online Access: | https://hdl.handle.net/1721.1/153501 |
| _version_ | 1811092419087695872 |
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| author | Thiabaud, Grégory D. Schwalm, Miriam Sen, Sajal Barandov, Ali Simon, Jacob Harvey, Peter Spanoudaki, Virginia Müller, Peter Sessler, Jonathan L. Jasanoff, Alan |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Thiabaud, Grégory D. Schwalm, Miriam Sen, Sajal Barandov, Ali Simon, Jacob Harvey, Peter Spanoudaki, Virginia Müller, Peter Sessler, Jonathan L. Jasanoff, Alan |
| author_sort | Thiabaud, Grégory D. |
| collection | MIT |
| description | The ability to monitor intracellular calcium concentrations using fluorescent probes has led to important insights into biological signaling processes at the cellular level. An important challenge is to relate such measurements to broader patterns of signaling across fields of view that are inaccessible to optical techniques. To meet this need, we synthesized molecular probes that couple calcium-binding moieties to lanthanide texaphyrins, resulting in complexes endowed with a diverse complement of magnetic and photophysical properties. We show that the probes permit intracellular calcium levels to be assessed by fluorescence, photoacoustic, and magnetic resonance imaging modalities and that they are detectable by multimodal imaging in brain tissue. This work thus establishes a route for monitoring signaling processes over a range of spatial and temporal scales. |
| first_indexed | 2024-09-23T15:17:52Z |
| format | Article |
| id | mit-1721.1/153501 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T15:17:52Z |
| publishDate | 2024 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1535012024-09-20T19:29:28Z Texaphyrin-Based Calcium Sensor for Multimodal Imaging Thiabaud, Grégory D. Schwalm, Miriam Sen, Sajal Barandov, Ali Simon, Jacob Harvey, Peter Spanoudaki, Virginia Müller, Peter Sessler, Jonathan L. Jasanoff, Alan Massachusetts Institute of Technology. Department of Biological Engineering Koch Institute for Integrative Cancer Research at MIT Massachusetts Institute of Technology. Department of Chemistry Fluid Flow and Transfer Processes Process Chemistry and Technology Instrumentation Bioengineering The ability to monitor intracellular calcium concentrations using fluorescent probes has led to important insights into biological signaling processes at the cellular level. An important challenge is to relate such measurements to broader patterns of signaling across fields of view that are inaccessible to optical techniques. To meet this need, we synthesized molecular probes that couple calcium-binding moieties to lanthanide texaphyrins, resulting in complexes endowed with a diverse complement of magnetic and photophysical properties. We show that the probes permit intracellular calcium levels to be assessed by fluorescence, photoacoustic, and magnetic resonance imaging modalities and that they are detectable by multimodal imaging in brain tissue. This work thus establishes a route for monitoring signaling processes over a range of spatial and temporal scales. National Institutes of Health (NIH) 2024-02-12T18:34:56Z 2024-02-12T18:34:56Z 2023-10-09 Article http://purl.org/eprint/type/JournalArticle 2379-3694 2379-3694 https://hdl.handle.net/1721.1/153501 Thiabaud, Grégory D., Schwalm, Miriam, Sen, Sajal, Barandov, Ali, Simon, Jacob et al. 2023. "Texaphyrin-Based Calcium Sensor for Multimodal Imaging." ACS Sensors, 8 (10). 10.1021/acssensors.3c01387 ACS Sensors Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Chemical Society (ACS) Author |
| spellingShingle | Fluid Flow and Transfer Processes Process Chemistry and Technology Instrumentation Bioengineering Thiabaud, Grégory D. Schwalm, Miriam Sen, Sajal Barandov, Ali Simon, Jacob Harvey, Peter Spanoudaki, Virginia Müller, Peter Sessler, Jonathan L. Jasanoff, Alan Texaphyrin-Based Calcium Sensor for Multimodal Imaging |
| title | Texaphyrin-Based Calcium Sensor for Multimodal Imaging |
| title_full | Texaphyrin-Based Calcium Sensor for Multimodal Imaging |
| title_fullStr | Texaphyrin-Based Calcium Sensor for Multimodal Imaging |
| title_full_unstemmed | Texaphyrin-Based Calcium Sensor for Multimodal Imaging |
| title_short | Texaphyrin-Based Calcium Sensor for Multimodal Imaging |
| title_sort | texaphyrin based calcium sensor for multimodal imaging |
| topic | Fluid Flow and Transfer Processes Process Chemistry and Technology Instrumentation Bioengineering |
| url | https://hdl.handle.net/1721.1/153501 |
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