Aphid Resistance Segregates Independently of Cardenolide and Glucosinolate Content in an Erysimum cheiranthoides (Wormseed Wallflower) F2 Population
Plants in the genus Erysimum produce both glucosinolates and cardenolides as a defense mechanism against herbivory. Two natural isolates of Erysimum cheiranthoides (wormseed wallflower) differed in their glucosinolate content, cardenolide content, and their resistance to Myzus persicae (green peach...
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| Format: | Article |
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MDPI AG
2024
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| Online Access: | https://hdl.handle.net/1721.1/153567 |
| _version_ | 1811075307956862976 |
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| author | Mirzaei, Mahdieh Younkin, Gordon C. Powell, Adrian F. Alani, Martin L. Strickler, Susan R. Jander, Georg |
| author_facet | Mirzaei, Mahdieh Younkin, Gordon C. Powell, Adrian F. Alani, Martin L. Strickler, Susan R. Jander, Georg |
| author_sort | Mirzaei, Mahdieh |
| collection | MIT |
| description | Plants in the genus Erysimum produce both glucosinolates and cardenolides as a defense mechanism against herbivory. Two natural isolates of Erysimum cheiranthoides (wormseed wallflower) differed in their glucosinolate content, cardenolide content, and their resistance to Myzus persicae (green peach aphid), a broad generalist herbivore. Both classes of defensive metabolites were produced constitutively and were not further induced by aphid feeding. To investigate the relative importance of glucosinolates and cardenolides in E. cheiranthoides defense, we generated an improved genome assembly, genetic map, and segregating F2 population. The genotypic and phenotypic analysis of the F2 plants identified quantitative trait loci, which affected glucosinolates and cardenolides, but not the aphid resistance. The abundance of most glucosinolates and cardenolides was positively correlated in the F2 population, indicating that similar processes regulate their biosynthesis and accumulation. Aphid reproduction was positively correlated with glucosinolate content. Although the overall cardenolide content had little effect on aphid growth and survival, there was a negative correlation between aphid reproduction and helveticoside abundance. However, this variation in defensive metabolites could not explain the differences in aphid growth on the two parental lines, suggesting that processes other than the abundance of glucosinolates and cardenolides have a predominant effect on aphid resistance in E. cheiranthoides. |
| first_indexed | 2024-09-23T10:03:59Z |
| format | Article |
| id | mit-1721.1/153567 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T10:03:59Z |
| publishDate | 2024 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | mit-1721.1/1535672024-02-24T03:51:47Z Aphid Resistance Segregates Independently of Cardenolide and Glucosinolate Content in an Erysimum cheiranthoides (Wormseed Wallflower) F2 Population Mirzaei, Mahdieh Younkin, Gordon C. Powell, Adrian F. Alani, Martin L. Strickler, Susan R. Jander, Georg Plant Science Ecology Ecology, Evolution, Behavior and Systematics Plants in the genus Erysimum produce both glucosinolates and cardenolides as a defense mechanism against herbivory. Two natural isolates of Erysimum cheiranthoides (wormseed wallflower) differed in their glucosinolate content, cardenolide content, and their resistance to Myzus persicae (green peach aphid), a broad generalist herbivore. Both classes of defensive metabolites were produced constitutively and were not further induced by aphid feeding. To investigate the relative importance of glucosinolates and cardenolides in E. cheiranthoides defense, we generated an improved genome assembly, genetic map, and segregating F2 population. The genotypic and phenotypic analysis of the F2 plants identified quantitative trait loci, which affected glucosinolates and cardenolides, but not the aphid resistance. The abundance of most glucosinolates and cardenolides was positively correlated in the F2 population, indicating that similar processes regulate their biosynthesis and accumulation. Aphid reproduction was positively correlated with glucosinolate content. Although the overall cardenolide content had little effect on aphid growth and survival, there was a negative correlation between aphid reproduction and helveticoside abundance. However, this variation in defensive metabolites could not explain the differences in aphid growth on the two parental lines, suggesting that processes other than the abundance of glucosinolates and cardenolides have a predominant effect on aphid resistance in E. cheiranthoides. 2024-02-23T18:58:31Z 2024-02-23T18:58:31Z 2024-02-06 2024-02-23T15:03:41Z Article http://purl.org/eprint/type/JournalArticle 2223-7747 https://hdl.handle.net/1721.1/153567 Mirzaei, M.; Younkin, G.C.; Powell, A.F.; Alani, M.L.; Strickler, S.R.; Jander, G. Aphid Resistance Segregates Independently of Cardenolide and Glucosinolate Content in an Erysimum cheiranthoides (Wormseed Wallflower) F2 Population. Plants 2024, 13, 466. 10.3390/plants13040466 Plants Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf MDPI AG Multidisciplinary Digital Publishing Institute |
| spellingShingle | Plant Science Ecology Ecology, Evolution, Behavior and Systematics Mirzaei, Mahdieh Younkin, Gordon C. Powell, Adrian F. Alani, Martin L. Strickler, Susan R. Jander, Georg Aphid Resistance Segregates Independently of Cardenolide and Glucosinolate Content in an Erysimum cheiranthoides (Wormseed Wallflower) F2 Population |
| title | Aphid Resistance Segregates Independently of Cardenolide and Glucosinolate Content in an Erysimum cheiranthoides (Wormseed Wallflower) F2 Population |
| title_full | Aphid Resistance Segregates Independently of Cardenolide and Glucosinolate Content in an Erysimum cheiranthoides (Wormseed Wallflower) F2 Population |
| title_fullStr | Aphid Resistance Segregates Independently of Cardenolide and Glucosinolate Content in an Erysimum cheiranthoides (Wormseed Wallflower) F2 Population |
| title_full_unstemmed | Aphid Resistance Segregates Independently of Cardenolide and Glucosinolate Content in an Erysimum cheiranthoides (Wormseed Wallflower) F2 Population |
| title_short | Aphid Resistance Segregates Independently of Cardenolide and Glucosinolate Content in an Erysimum cheiranthoides (Wormseed Wallflower) F2 Population |
| title_sort | aphid resistance segregates independently of cardenolide and glucosinolate content in an erysimum cheiranthoides wormseed wallflower f2 population |
| topic | Plant Science Ecology Ecology, Evolution, Behavior and Systematics |
| url | https://hdl.handle.net/1721.1/153567 |
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