Optimization of 3D autologous chondrocyte-seeded polyglycolic acid scaffolds to mimic human ear cartilage
Auricular reconstruction in children with microtia is one of the more complex procedures in plastic surgery. Obtaining sufficient native material to build an ear requires harvesting large fragments of rib cartilage in children. Herein, we investigated how to optimize autologous chondrocyte isolation...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
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Royal Society of Chemistry
2024
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| Online Access: | https://hdl.handle.net/1721.1/154128 |
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| author | Melgar-Lesmes, Pedro Bosch, Oriol Zubajlo, Rebecca Molins, Gemma Comfort, Sofia Luque-Saavedra, Ainara López-Moya, Mario García-Polite, Fernando Parri Ferrandis, Francisco José Rogers, Carolyn Gelabertó, Agata Martorell, Jordi Edelman, Elazer R. Balcells, Mercedes |
| author_facet | Melgar-Lesmes, Pedro Bosch, Oriol Zubajlo, Rebecca Molins, Gemma Comfort, Sofia Luque-Saavedra, Ainara López-Moya, Mario García-Polite, Fernando Parri Ferrandis, Francisco José Rogers, Carolyn Gelabertó, Agata Martorell, Jordi Edelman, Elazer R. Balcells, Mercedes |
| author_sort | Melgar-Lesmes, Pedro |
| collection | MIT |
| description | Auricular reconstruction in children with microtia is one of the more complex procedures in plastic surgery. Obtaining sufficient native material to build an ear requires harvesting large fragments of rib cartilage in children. Herein, we investigated how to optimize autologous chondrocyte isolation, expansion and re-implantation using polyglycolic acid (PGA) scaffolds for generating enough cartilage to recapitulate a whole ear starting from a small ear biopsy. Ear chondrocytes isolated from human microtia subjects grew slower than microtia rib or healthy ear chondrocytes and displayed a phenotypic shift due to the passage number. Rabbit ear chondrocytes co-cultured with mesenchymal stem cells (MSC) at a 50 : 50 ratio recapitulated the cartilage biological properties in vitro. However, PGA scaffolds with different proportions of rabbit chondrocytes and MSC did not grow substantially in two months when subcutaneously implanted in immunosuppressed mice. In contrast, rabbit chondrocyte-seeded PGA scaffolds implanted in immunocompetent rabbits formed a cartilage 10 times larger than the original PGA scaffold. This cartilage mimicked the biofunctional and mechanical properties of an ear cartilage. These results indicate that autologous chondrocyte-seeded PGA scaffolds fabricated following our optimized procedure have immense potential as a solution for obtaining enough cartilage for auricular reconstruction and opens new avenues to redefine autologous cartilage replacement. |
| first_indexed | 2024-09-23T16:57:10Z |
| format | Article |
| id | mit-1721.1/154128 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T16:57:10Z |
| publishDate | 2024 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/1541282024-09-10T04:17:55Z Optimization of 3D autologous chondrocyte-seeded polyglycolic acid scaffolds to mimic human ear cartilage Melgar-Lesmes, Pedro Bosch, Oriol Zubajlo, Rebecca Molins, Gemma Comfort, Sofia Luque-Saavedra, Ainara López-Moya, Mario García-Polite, Fernando Parri Ferrandis, Francisco José Rogers, Carolyn Gelabertó, Agata Martorell, Jordi Edelman, Elazer R. Balcells, Mercedes General Materials Science Biomedical Engineering Auricular reconstruction in children with microtia is one of the more complex procedures in plastic surgery. Obtaining sufficient native material to build an ear requires harvesting large fragments of rib cartilage in children. Herein, we investigated how to optimize autologous chondrocyte isolation, expansion and re-implantation using polyglycolic acid (PGA) scaffolds for generating enough cartilage to recapitulate a whole ear starting from a small ear biopsy. Ear chondrocytes isolated from human microtia subjects grew slower than microtia rib or healthy ear chondrocytes and displayed a phenotypic shift due to the passage number. Rabbit ear chondrocytes co-cultured with mesenchymal stem cells (MSC) at a 50 : 50 ratio recapitulated the cartilage biological properties in vitro. However, PGA scaffolds with different proportions of rabbit chondrocytes and MSC did not grow substantially in two months when subcutaneously implanted in immunosuppressed mice. In contrast, rabbit chondrocyte-seeded PGA scaffolds implanted in immunocompetent rabbits formed a cartilage 10 times larger than the original PGA scaffold. This cartilage mimicked the biofunctional and mechanical properties of an ear cartilage. These results indicate that autologous chondrocyte-seeded PGA scaffolds fabricated following our optimized procedure have immense potential as a solution for obtaining enough cartilage for auricular reconstruction and opens new avenues to redefine autologous cartilage replacement. European Regional Development Fund; Instituto de Salud Carlos III; Ministerio de Ciencia e Innovación; National Institutes of Health 2024-04-12T14:24:12Z 2024-04-12T14:24:12Z 2023 Article http://purl.org/eprint/type/JournalArticle 2047-4830 2047-4849 https://hdl.handle.net/1721.1/154128 Melgar-Lesmes, Pedro, Bosch, Oriol, Zubajlo, Rebecca, Molins, Gemma, Comfort, Sofia et al. 2023. "Optimization of 3D autologous chondrocyte-seeded polyglycolic acid scaffolds to mimic human ear cartilage." Biomaterials Science, 11 (10). PUBLISHER_CC 10.1039/d3bm00035d Biomaterials Science Creative Commons Attribution https://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry Royal Society of Chemistry |
| spellingShingle | General Materials Science Biomedical Engineering Melgar-Lesmes, Pedro Bosch, Oriol Zubajlo, Rebecca Molins, Gemma Comfort, Sofia Luque-Saavedra, Ainara López-Moya, Mario García-Polite, Fernando Parri Ferrandis, Francisco José Rogers, Carolyn Gelabertó, Agata Martorell, Jordi Edelman, Elazer R. Balcells, Mercedes Optimization of 3D autologous chondrocyte-seeded polyglycolic acid scaffolds to mimic human ear cartilage |
| title | Optimization of 3D autologous chondrocyte-seeded polyglycolic acid scaffolds to mimic human ear cartilage |
| title_full | Optimization of 3D autologous chondrocyte-seeded polyglycolic acid scaffolds to mimic human ear cartilage |
| title_fullStr | Optimization of 3D autologous chondrocyte-seeded polyglycolic acid scaffolds to mimic human ear cartilage |
| title_full_unstemmed | Optimization of 3D autologous chondrocyte-seeded polyglycolic acid scaffolds to mimic human ear cartilage |
| title_short | Optimization of 3D autologous chondrocyte-seeded polyglycolic acid scaffolds to mimic human ear cartilage |
| title_sort | optimization of 3d autologous chondrocyte seeded polyglycolic acid scaffolds to mimic human ear cartilage |
| topic | General Materials Science Biomedical Engineering |
| url | https://hdl.handle.net/1721.1/154128 |
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