Zeta potential characterization using commercial microfluidic chips
Surface charge is a critical feature of microbes that affects their interactions with other cells and their environment. Because bacterial surface charge is difficult to measure directly, it is typically indirectly inferred through zeta potential measurements. Existing tools to perform such characte...
| Main Authors: | , , , , , |
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| Format: | Article |
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Royal Society of Chemistry
2024
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| Online Access: | https://hdl.handle.net/1721.1/154140 |
| _version_ | 1826205523012222976 |
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| author | Cottet, Jonathan Oshodi, Josephine O. Yebouet, Jesse Leang, Andrea Furst, Ariel L. Buie, Cullen R. |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Cottet, Jonathan Oshodi, Josephine O. Yebouet, Jesse Leang, Andrea Furst, Ariel L. Buie, Cullen R. |
| author_sort | Cottet, Jonathan |
| collection | MIT |
| description | Surface charge is a critical feature of microbes that affects their interactions with other cells and their environment. Because bacterial surface charge is difficult to measure directly, it is typically indirectly inferred through zeta potential measurements. Existing tools to perform such characterization are either costly and ill-suited for non-spherical samples or rely on microfluidic techniques requiring expensive fabrication equipment or specialized facilities. Here, we report the application of commercially available PMMA microfluidic chips and open-source data analysis workflows for facile electrokinetic characterization of particles and cells after prior zeta potential measurement with a Zetasizer for calibration. Our workflows eliminate the need for microchannel fabrication, increase measurement reproducibility, and make zeta potential measurements more accessible. This novel methodology was tested with functionalized 1 μm and 2 μm polystyrene beads as well as Escherichia coli MG1655 strain. Measured zeta potentials for these samples were in agreement with literature values obtained by conventional measurement methods. Taken together, our data demonstrate the power of this workflow to broadly enable critical measurements of particle and bacterial zeta potential for numerous applications. |
| first_indexed | 2024-09-23T13:14:49Z |
| format | Article |
| id | mit-1721.1/154140 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2025-02-19T04:22:06Z |
| publishDate | 2024 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/1541402025-01-08T04:44:31Z Zeta potential characterization using commercial microfluidic chips Cottet, Jonathan Oshodi, Josephine O. Yebouet, Jesse Leang, Andrea Furst, Ariel L. Buie, Cullen R. Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Center for Environmental Health Sciences Biomedical Engineering General Chemistry Biochemistry Bioengineering Surface charge is a critical feature of microbes that affects their interactions with other cells and their environment. Because bacterial surface charge is difficult to measure directly, it is typically indirectly inferred through zeta potential measurements. Existing tools to perform such characterization are either costly and ill-suited for non-spherical samples or rely on microfluidic techniques requiring expensive fabrication equipment or specialized facilities. Here, we report the application of commercially available PMMA microfluidic chips and open-source data analysis workflows for facile electrokinetic characterization of particles and cells after prior zeta potential measurement with a Zetasizer for calibration. Our workflows eliminate the need for microchannel fabrication, increase measurement reproducibility, and make zeta potential measurements more accessible. This novel methodology was tested with functionalized 1 μm and 2 μm polystyrene beads as well as Escherichia coli MG1655 strain. Measured zeta potentials for these samples were in agreement with literature values obtained by conventional measurement methods. Taken together, our data demonstrate the power of this workflow to broadly enable critical measurements of particle and bacterial zeta potential for numerous applications. Office of Naval Research Global 2024-04-12T17:57:38Z 2024-04-12T17:57:38Z 2024 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 https://hdl.handle.net/1721.1/154140 Cottet, Jonathan, Oshodi, Josephine O., Yebouet, Jesse, Leang, Andrea, Furst, Ariel L. et al. 2024. "Zeta potential characterization using commercial microfluidic chips." Lab on a Chip, 24 (2). PUBLISHER_CC 10.1039/d3lc00825h Lab on a Chip Creative Commons Attribution https://creativecommons.org/licenses/by/3.0/ application/pdf Royal Society of Chemistry Royal Society of Chemistry |
| spellingShingle | Biomedical Engineering General Chemistry Biochemistry Bioengineering Cottet, Jonathan Oshodi, Josephine O. Yebouet, Jesse Leang, Andrea Furst, Ariel L. Buie, Cullen R. Zeta potential characterization using commercial microfluidic chips |
| title | Zeta potential characterization using commercial microfluidic chips |
| title_full | Zeta potential characterization using commercial microfluidic chips |
| title_fullStr | Zeta potential characterization using commercial microfluidic chips |
| title_full_unstemmed | Zeta potential characterization using commercial microfluidic chips |
| title_short | Zeta potential characterization using commercial microfluidic chips |
| title_sort | zeta potential characterization using commercial microfluidic chips |
| topic | Biomedical Engineering General Chemistry Biochemistry Bioengineering |
| url | https://hdl.handle.net/1721.1/154140 |
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