Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting
The growing interest in regenerative medicine has opened new avenues for novel cell therapies using stem cells. Bone marrow aspirate (BMA) is an important source of stromal mesenchymal stem cells (MSCs). Conventional MSC harvesting from BMA relies on archaic centrifugation methods, often leading to...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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Royal Society of Chemistry
2024
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| Online Access: | https://hdl.handle.net/1721.1/154144 |
| _version_ | 1811090803814039552 |
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| author | Tan Kwan Zen, Nicholas Zeming, Kerwin Kwek Teo, Kim Leng Loberas, Mavis Lee, Jialing Goh, Chin Ren Yang, Da Hou Oh, Steve Hui Hoi Po, James Cool, Simon M. Hou, Han Wei Han, Jongyoon |
| author_facet | Tan Kwan Zen, Nicholas Zeming, Kerwin Kwek Teo, Kim Leng Loberas, Mavis Lee, Jialing Goh, Chin Ren Yang, Da Hou Oh, Steve Hui Hoi Po, James Cool, Simon M. Hou, Han Wei Han, Jongyoon |
| author_sort | Tan Kwan Zen, Nicholas |
| collection | MIT |
| description | The growing interest in regenerative medicine has opened new avenues for novel cell therapies using stem cells. Bone marrow aspirate (BMA) is an important source of stromal mesenchymal stem cells (MSCs). Conventional MSC harvesting from BMA relies on archaic centrifugation methods, often leading to poor yield due to osmotic stress, high centrifugation force, convoluted workflow, and long experimental time (∼2–3 hours). To address these issues, we have developed a scalable microfluidic technology based on deterministic lateral displacement (DLD) for MSC isolation. This passive, label-free cell sorting method capitalizes on the morphological differences between MSCs and blood cells (platelets and RBCs) for effective separation using an inverted L-shaped pillar array. To improve throughput, we developed a novel multi-chip DLD system that can process 2.5 mL of raw BMA in 20 ± 5 minutes, achieving a 2-fold increase in MSC recovery compared to centrifugation methods. Taken together, we envision that the developed DLD platform will enable fast and efficient isolation of MSCs from BMA for effective downstream cell therapy in clinical settings. |
| first_indexed | 2024-09-23T14:51:54Z |
| format | Article |
| id | mit-1721.1/154144 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T14:51:54Z |
| publishDate | 2024 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/1541442024-09-10T04:33:19Z Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting Tan Kwan Zen, Nicholas Zeming, Kerwin Kwek Teo, Kim Leng Loberas, Mavis Lee, Jialing Goh, Chin Ren Yang, Da Hou Oh, Steve Hui Hoi Po, James Cool, Simon M. Hou, Han Wei Han, Jongyoon Biomedical Engineering General Chemistry Biochemistry Bioengineering The growing interest in regenerative medicine has opened new avenues for novel cell therapies using stem cells. Bone marrow aspirate (BMA) is an important source of stromal mesenchymal stem cells (MSCs). Conventional MSC harvesting from BMA relies on archaic centrifugation methods, often leading to poor yield due to osmotic stress, high centrifugation force, convoluted workflow, and long experimental time (∼2–3 hours). To address these issues, we have developed a scalable microfluidic technology based on deterministic lateral displacement (DLD) for MSC isolation. This passive, label-free cell sorting method capitalizes on the morphological differences between MSCs and blood cells (platelets and RBCs) for effective separation using an inverted L-shaped pillar array. To improve throughput, we developed a novel multi-chip DLD system that can process 2.5 mL of raw BMA in 20 ± 5 minutes, achieving a 2-fold increase in MSC recovery compared to centrifugation methods. Taken together, we envision that the developed DLD platform will enable fast and efficient isolation of MSCs from BMA for effective downstream cell therapy in clinical settings. National Research Foundation Singapore 2024-04-12T18:20:10Z 2024-04-12T18:20:10Z 2023 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 https://hdl.handle.net/1721.1/154144 Tan Kwan Zen, Nicholas, Zeming, Kerwin Kwek, Teo, Kim Leng, Loberas, Mavis, Lee, Jialing et al. 2023. "Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting." Lab on a Chip, 23 (19). PUBLISHER_CC 10.1039/d3lc00379e Lab on a Chip Creative Commons Attribution https://creativecommons.org/licenses/by/3.0/ application/pdf Royal Society of Chemistry Royal Society of Chemistry |
| spellingShingle | Biomedical Engineering General Chemistry Biochemistry Bioengineering Tan Kwan Zen, Nicholas Zeming, Kerwin Kwek Teo, Kim Leng Loberas, Mavis Lee, Jialing Goh, Chin Ren Yang, Da Hou Oh, Steve Hui Hoi Po, James Cool, Simon M. Hou, Han Wei Han, Jongyoon Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting |
| title | Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting |
| title_full | Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting |
| title_fullStr | Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting |
| title_full_unstemmed | Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting |
| title_short | Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting |
| title_sort | scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement dld microfluidic sorting |
| topic | Biomedical Engineering General Chemistry Biochemistry Bioengineering |
| url | https://hdl.handle.net/1721.1/154144 |
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