Optical diffraction tomography for assessing single cell models in angular light scattering
Angularly resolved light scattering (ALS) has become a useful tool for assessing the size and refractive index of biological scatterers at cellular and organelle length scales. Sizing organelle populations with ALS relies on Mie scattering theory models, which require significant assumptions about t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Optica Publishing Group
2024
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| Online Access: | https://hdl.handle.net/1721.1/154312 |
| _version_ | 1811087484490088448 |
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| author | Dunn, Kaitlin J. Matlock, Alex Funkenbusch, George Yaqoob, Zahid So, Peter T. C. Berger, Andrew J. |
| author_facet | Dunn, Kaitlin J. Matlock, Alex Funkenbusch, George Yaqoob, Zahid So, Peter T. C. Berger, Andrew J. |
| author_sort | Dunn, Kaitlin J. |
| collection | MIT |
| description | Angularly resolved light scattering (ALS) has become a useful tool for assessing the size and refractive index of biological scatterers at cellular and organelle length scales. Sizing organelle populations with ALS relies on Mie scattering theory models, which require significant assumptions about the object, including spherical scatterers and a homogeneous medium. These assumptions may incur greater error at the single cell level, where there are fewer scatterers to be averaged over. We investigate the validity of these assumptions using 3D refractive index (RI) tomograms measured via optical diffraction tomography (ODT). We compute the angular scattering on digitally manipulated tomograms with increasingly strong model assumptions, including RI-matched immersion media, homogeneous cytosol, and spherical organelles. We also compare the tomogram-computed angular scattering to experimental measurements of angular scattering from the same cells to ensure that the ODT-based approach accurately models angular scattering. We show that enforced RI-matching with the immersion medium and a homogeneous cytosol significantly affects the angular scattering intensity shape, suggesting that these assumptions can reduce the accuracy of size distribution estimates. |
| first_indexed | 2024-09-23T13:46:32Z |
| format | Article |
| id | mit-1721.1/154312 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:46:32Z |
| publishDate | 2024 |
| publisher | Optica Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1543122024-09-18T05:04:27Z Optical diffraction tomography for assessing single cell models in angular light scattering Dunn, Kaitlin J. Matlock, Alex Funkenbusch, George Yaqoob, Zahid So, Peter T. C. Berger, Andrew J. Angularly resolved light scattering (ALS) has become a useful tool for assessing the size and refractive index of biological scatterers at cellular and organelle length scales. Sizing organelle populations with ALS relies on Mie scattering theory models, which require significant assumptions about the object, including spherical scatterers and a homogeneous medium. These assumptions may incur greater error at the single cell level, where there are fewer scatterers to be averaged over. We investigate the validity of these assumptions using 3D refractive index (RI) tomograms measured via optical diffraction tomography (ODT). We compute the angular scattering on digitally manipulated tomograms with increasingly strong model assumptions, including RI-matched immersion media, homogeneous cytosol, and spherical organelles. We also compare the tomogram-computed angular scattering to experimental measurements of angular scattering from the same cells to ensure that the ODT-based approach accurately models angular scattering. We show that enforced RI-matching with the immersion medium and a homogeneous cytosol significantly affects the angular scattering intensity shape, suggesting that these assumptions can reduce the accuracy of size distribution estimates. 2024-04-29T20:10:44Z 2024-04-29T20:10:44Z 2024-01-24 2024-04-29T20:04:25Z Article http://purl.org/eprint/type/JournalArticle 2156-7085 2156-7085 https://hdl.handle.net/1721.1/154312 Kaitlin J. Dunn, Alex Matlock, George Funkenbusch, Zahid Yaqoob, Peter T. C. So, and Andrew J. Berger, "Optical diffraction tomography for assessing single cell models in angular light scattering," Biomed. Opt. Express 15, 973-990 (2024) en 10.1364/boe.512149 Biomedical Optics Express Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Optica Publishing Group Optica Publishing Group |
| spellingShingle | Dunn, Kaitlin J. Matlock, Alex Funkenbusch, George Yaqoob, Zahid So, Peter T. C. Berger, Andrew J. Optical diffraction tomography for assessing single cell models in angular light scattering |
| title | Optical diffraction tomography for assessing single cell models in angular light scattering |
| title_full | Optical diffraction tomography for assessing single cell models in angular light scattering |
| title_fullStr | Optical diffraction tomography for assessing single cell models in angular light scattering |
| title_full_unstemmed | Optical diffraction tomography for assessing single cell models in angular light scattering |
| title_short | Optical diffraction tomography for assessing single cell models in angular light scattering |
| title_sort | optical diffraction tomography for assessing single cell models in angular light scattering |
| url | https://hdl.handle.net/1721.1/154312 |
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