Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography
Resolving three-dimensional morphological features in thick specimens remains a significant challenge for label-free imaging. We report a new speckle diffraction tomography (SDT) approach that can image thick biological specimens with ~500 nm lateral resolution and ~1 μm axial resolution in a reflec...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2024
|
| Online Access: | https://hdl.handle.net/1721.1/154314 |
| _version_ | 1811098239192006656 |
|---|---|
| author | Kang, Sungsam Zhou, Renjie Brelen, Marten Mak, Heather K. Lin, Yuechuan So, Peter T. C. Yaqoob, Zahid |
| author_facet | Kang, Sungsam Zhou, Renjie Brelen, Marten Mak, Heather K. Lin, Yuechuan So, Peter T. C. Yaqoob, Zahid |
| author_sort | Kang, Sungsam |
| collection | MIT |
| description | Resolving three-dimensional morphological features in thick specimens remains a significant challenge for label-free imaging. We report a new speckle diffraction tomography (SDT) approach that can image thick biological specimens with ~500 nm lateral resolution and ~1 μm axial resolution in a reflection geometry. In SDT, multiple-scattering background is rejected through spatiotemporal gating provided by dynamic speckle-field interferometry, while depth-resolved refractive index maps are reconstructed by developing a comprehensive inverse-scattering model that also considers specimen-induced aberrations. Benefiting from the high-resolution and full-field quantitative imaging capabilities of SDT, we successfully imaged red blood cells and quantified their membrane fluctuations behind a turbid medium with a thickness of 2.8 scattering mean-free paths. Most importantly, we performed volumetric imaging of cornea inside an ex vivo rat eye and quantified its optical properties, including the mapping of nanoscale topographic features of Dua’s and Descemet’s membranes that had not been previously visualized. |
| first_indexed | 2024-09-23T17:12:14Z |
| format | Article |
| id | mit-1721.1/154314 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T17:12:14Z |
| publishDate | 2024 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1543142024-09-16T04:11:59Z Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography Kang, Sungsam Zhou, Renjie Brelen, Marten Mak, Heather K. Lin, Yuechuan So, Peter T. C. Yaqoob, Zahid Resolving three-dimensional morphological features in thick specimens remains a significant challenge for label-free imaging. We report a new speckle diffraction tomography (SDT) approach that can image thick biological specimens with ~500 nm lateral resolution and ~1 μm axial resolution in a reflection geometry. In SDT, multiple-scattering background is rejected through spatiotemporal gating provided by dynamic speckle-field interferometry, while depth-resolved refractive index maps are reconstructed by developing a comprehensive inverse-scattering model that also considers specimen-induced aberrations. Benefiting from the high-resolution and full-field quantitative imaging capabilities of SDT, we successfully imaged red blood cells and quantified their membrane fluctuations behind a turbid medium with a thickness of 2.8 scattering mean-free paths. Most importantly, we performed volumetric imaging of cornea inside an ex vivo rat eye and quantified its optical properties, including the mapping of nanoscale topographic features of Dua’s and Descemet’s membranes that had not been previously visualized. 2024-04-30T18:07:23Z 2024-04-30T18:07:23Z 2023-08-22 2024-04-30T18:03:22Z Article http://purl.org/eprint/type/JournalArticle 2047-7538 https://hdl.handle.net/1721.1/154314 Kang, S., Zhou, R., Brelen, M. et al. Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography. Light Sci Appl 12, 200 (2023). en 10.1038/s41377-023-01240-0 Light: Science & Applications Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Springer Science and Business Media LLC |
| spellingShingle | Kang, Sungsam Zhou, Renjie Brelen, Marten Mak, Heather K. Lin, Yuechuan So, Peter T. C. Yaqoob, Zahid Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography |
| title | Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography |
| title_full | Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography |
| title_fullStr | Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography |
| title_full_unstemmed | Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography |
| title_short | Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography |
| title_sort | mapping nanoscale topographic features in thick tissues with speckle diffraction tomography |
| url | https://hdl.handle.net/1721.1/154314 |
| work_keys_str_mv | AT kangsungsam mappingnanoscaletopographicfeaturesinthicktissueswithspecklediffractiontomography AT zhourenjie mappingnanoscaletopographicfeaturesinthicktissueswithspecklediffractiontomography AT brelenmarten mappingnanoscaletopographicfeaturesinthicktissueswithspecklediffractiontomography AT makheatherk mappingnanoscaletopographicfeaturesinthicktissueswithspecklediffractiontomography AT linyuechuan mappingnanoscaletopographicfeaturesinthicktissueswithspecklediffractiontomography AT sopetertc mappingnanoscaletopographicfeaturesinthicktissueswithspecklediffractiontomography AT yaqoobzahid mappingnanoscaletopographicfeaturesinthicktissueswithspecklediffractiontomography |