Biofilm formation of Pseudomonas aeruginosa in spaceflight is minimized on lubricant impregnated surfaces
The undesirable, yet inevitable, presence of bacterial biofilms in spacecraft poses a risk to the proper functioning of systems and to astronauts’ health. To mitigate the risks that arise from them, it is important to understand biofilms’ behavior in microgravity. As part of the Space Biofilms proje...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
2024
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| Online Access: | https://hdl.handle.net/1721.1/154925 |
| _version_ | 1811071284208992256 |
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| author | Flores, Pamela McBride, Samantha A. Galazka, Jonathan M. Varanasi, Kripa K. Zea, Luis |
| author_facet | Flores, Pamela McBride, Samantha A. Galazka, Jonathan M. Varanasi, Kripa K. Zea, Luis |
| author_sort | Flores, Pamela |
| collection | MIT |
| description | The undesirable, yet inevitable, presence of bacterial biofilms in spacecraft poses a risk to the proper functioning of systems and to astronauts’ health. To mitigate the risks that arise from them, it is important to understand biofilms’ behavior in microgravity. As part of the Space Biofilms project, biofilms of <jats:italic>Pseudomonas aeruginosa</jats:italic> were grown in spaceflight over material surfaces. Stainless Steel 316 (SS316) and passivated SS316 were tested for their relevance as spaceflight hardware components, while a lubricant impregnated surface (LIS) was tested as potential biofilm control strategy. The morphology and gene expression of biofilms were characterized. Biofilms in microgravity are less robust than on Earth. LIS strongly inhibits biofilm formation compared to SS. Furthermore, this effect is even greater in spaceflight than on Earth, making LIS a promising option for spacecraft use. Transcriptomic profiles for the different conditions are presented, and potential mechanisms of biofilm reduction on LIS are discussed. |
| first_indexed | 2024-09-23T08:48:45Z |
| format | Article |
| id | mit-1721.1/154925 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T08:48:45Z |
| publishDate | 2024 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1549252024-05-11T03:59:53Z Biofilm formation of Pseudomonas aeruginosa in spaceflight is minimized on lubricant impregnated surfaces Flores, Pamela McBride, Samantha A. Galazka, Jonathan M. Varanasi, Kripa K. Zea, Luis The undesirable, yet inevitable, presence of bacterial biofilms in spacecraft poses a risk to the proper functioning of systems and to astronauts’ health. To mitigate the risks that arise from them, it is important to understand biofilms’ behavior in microgravity. As part of the Space Biofilms project, biofilms of <jats:italic>Pseudomonas aeruginosa</jats:italic> were grown in spaceflight over material surfaces. Stainless Steel 316 (SS316) and passivated SS316 were tested for their relevance as spaceflight hardware components, while a lubricant impregnated surface (LIS) was tested as potential biofilm control strategy. The morphology and gene expression of biofilms were characterized. Biofilms in microgravity are less robust than on Earth. LIS strongly inhibits biofilm formation compared to SS. Furthermore, this effect is even greater in spaceflight than on Earth, making LIS a promising option for spacecraft use. Transcriptomic profiles for the different conditions are presented, and potential mechanisms of biofilm reduction on LIS are discussed. 2024-05-10T21:00:32Z 2024-05-10T21:00:32Z 2023-08-16 2024-05-10T20:56:57Z Article http://purl.org/eprint/type/JournalArticle 2373-8065 https://hdl.handle.net/1721.1/154925 Flores, P., McBride, S.A., Galazka, J.M. et al. Biofilm formation of Pseudomonas aeruginosa in spaceflight is minimized on lubricant impregnated surfaces. npj Microgravity 9, 66 (2023). en 10.1038/s41526-023-00316-w npj Microgravity Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Science and Business Media LLC Springer Science and Business Media LLC |
| spellingShingle | Flores, Pamela McBride, Samantha A. Galazka, Jonathan M. Varanasi, Kripa K. Zea, Luis Biofilm formation of Pseudomonas aeruginosa in spaceflight is minimized on lubricant impregnated surfaces |
| title | Biofilm formation of Pseudomonas aeruginosa in spaceflight is minimized on lubricant impregnated surfaces |
| title_full | Biofilm formation of Pseudomonas aeruginosa in spaceflight is minimized on lubricant impregnated surfaces |
| title_fullStr | Biofilm formation of Pseudomonas aeruginosa in spaceflight is minimized on lubricant impregnated surfaces |
| title_full_unstemmed | Biofilm formation of Pseudomonas aeruginosa in spaceflight is minimized on lubricant impregnated surfaces |
| title_short | Biofilm formation of Pseudomonas aeruginosa in spaceflight is minimized on lubricant impregnated surfaces |
| title_sort | biofilm formation of pseudomonas aeruginosa in spaceflight is minimized on lubricant impregnated surfaces |
| url | https://hdl.handle.net/1721.1/154925 |
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