Internal initiation of reverse transcription in a Penelope-like retrotransposon
Eukaryotic retroelements are generally divided into two classes: long terminal repeat (LTR) retrotransposons and non-LTR retrotransposons. A third class of eukaryotic retroelement, the Penelope-like elements (PLEs), has been well-characterized bioinformatically, but relatively little is known about...
| Main Authors: | , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2024
|
| Online Access: | https://hdl.handle.net/1721.1/155283 |
| _version_ | 1824458060076154880 |
|---|---|
| author | Frangieh, Chris J. Wilkinson, Max E. Strebinger, Daniel Strecker, Jonathan Walsh, Michelle L. Faure, Guilhem Yushenova, Irina A. Macrae, Rhiannon K. Arkhipova, Irina R. Zhang, Feng |
| author2 | McGovern Institute for Brain Research at MIT |
| author_facet | McGovern Institute for Brain Research at MIT Frangieh, Chris J. Wilkinson, Max E. Strebinger, Daniel Strecker, Jonathan Walsh, Michelle L. Faure, Guilhem Yushenova, Irina A. Macrae, Rhiannon K. Arkhipova, Irina R. Zhang, Feng |
| author_sort | Frangieh, Chris J. |
| collection | MIT |
| description | Eukaryotic retroelements are generally divided into two classes: long terminal repeat (LTR) retrotransposons and non-LTR retrotransposons. A third class of eukaryotic retroelement, the Penelope-like elements (PLEs), has been well-characterized bioinformatically, but relatively little is known about the transposition mechanism of these elements. PLEs share some features with the R2 retrotransposon from Bombyx mori, which uses a target-primed reverse transcription (TPRT) mechanism, but their distinct phylogeny suggests PLEs may utilize a novel mechanism of mobilization. Using protein purified from E. coli, we report unique in vitro properties of a PLE from the green anole (Anolis carolinensis), revealing mechanistic aspects not shared by other retrotransposons. We found that reverse transcription is initiated at two adjacent sites within the transposon RNA that is not homologous to the cleaved DNA, a feature that is reflected in the genomic “tail” signature shared between and unique to PLEs. Our results for the first active PLE in vitro provide a starting point for understanding PLE mobilization and biology. |
| first_indexed | 2024-09-23T11:06:41Z |
| format | Article |
| id | mit-1721.1/155283 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2025-02-19T04:19:53Z |
| publishDate | 2024 |
| publisher | Springer Science and Business Media LLC |
| record_format | dspace |
| spelling | mit-1721.1/1552832024-12-21T05:49:21Z Internal initiation of reverse transcription in a Penelope-like retrotransposon Frangieh, Chris J. Wilkinson, Max E. Strebinger, Daniel Strecker, Jonathan Walsh, Michelle L. Faure, Guilhem Yushenova, Irina A. Macrae, Rhiannon K. Arkhipova, Irina R. Zhang, Feng McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Howard Hughes Medical Institute Woods Hole Oceanographic Institution Eukaryotic retroelements are generally divided into two classes: long terminal repeat (LTR) retrotransposons and non-LTR retrotransposons. A third class of eukaryotic retroelement, the Penelope-like elements (PLEs), has been well-characterized bioinformatically, but relatively little is known about the transposition mechanism of these elements. PLEs share some features with the R2 retrotransposon from Bombyx mori, which uses a target-primed reverse transcription (TPRT) mechanism, but their distinct phylogeny suggests PLEs may utilize a novel mechanism of mobilization. Using protein purified from E. coli, we report unique in vitro properties of a PLE from the green anole (Anolis carolinensis), revealing mechanistic aspects not shared by other retrotransposons. We found that reverse transcription is initiated at two adjacent sites within the transposon RNA that is not homologous to the cleaved DNA, a feature that is reflected in the genomic “tail” signature shared between and unique to PLEs. Our results for the first active PLE in vitro provide a starting point for understanding PLE mobilization and biology. 2024-06-17T18:01:05Z 2024-06-17T18:01:05Z 2024-06-11 2024-06-16T03:13:15Z Article http://purl.org/eprint/type/JournalArticle 1759-8753 https://hdl.handle.net/1721.1/155283 Frangieh, C.J., Wilkinson, M.E., Strebinger, D. et al. Internal initiation of reverse transcription in a Penelope-like retrotransposon. Mobile DNA 15, 12 (2024). PUBLISHER_CC en 10.1186/s13100-024-00322-z Mobile DNA Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ The Author(s) application/pdf Springer Science and Business Media LLC BioMed Central |
| spellingShingle | Frangieh, Chris J. Wilkinson, Max E. Strebinger, Daniel Strecker, Jonathan Walsh, Michelle L. Faure, Guilhem Yushenova, Irina A. Macrae, Rhiannon K. Arkhipova, Irina R. Zhang, Feng Internal initiation of reverse transcription in a Penelope-like retrotransposon |
| title | Internal initiation of reverse transcription in a Penelope-like retrotransposon |
| title_full | Internal initiation of reverse transcription in a Penelope-like retrotransposon |
| title_fullStr | Internal initiation of reverse transcription in a Penelope-like retrotransposon |
| title_full_unstemmed | Internal initiation of reverse transcription in a Penelope-like retrotransposon |
| title_short | Internal initiation of reverse transcription in a Penelope-like retrotransposon |
| title_sort | internal initiation of reverse transcription in a penelope like retrotransposon |
| url | https://hdl.handle.net/1721.1/155283 |
| work_keys_str_mv | AT frangiehchrisj internalinitiationofreversetranscriptioninapenelopelikeretrotransposon AT wilkinsonmaxe internalinitiationofreversetranscriptioninapenelopelikeretrotransposon AT strebingerdaniel internalinitiationofreversetranscriptioninapenelopelikeretrotransposon AT streckerjonathan internalinitiationofreversetranscriptioninapenelopelikeretrotransposon AT walshmichellel internalinitiationofreversetranscriptioninapenelopelikeretrotransposon AT faureguilhem internalinitiationofreversetranscriptioninapenelopelikeretrotransposon AT yushenovairinaa internalinitiationofreversetranscriptioninapenelopelikeretrotransposon AT macraerhiannonk internalinitiationofreversetranscriptioninapenelopelikeretrotransposon AT arkhipovairinar internalinitiationofreversetranscriptioninapenelopelikeretrotransposon AT zhangfeng internalinitiationofreversetranscriptioninapenelopelikeretrotransposon |