QuickFit: A High-Throughput RT-qPCR-Based Assay to Quantify Viral Growth and Fitness In Vitro
Quantifying viral growth rates is key to understanding evolutionary dynamics and the potential for mutants to escape antiviral drugs. Defining evolutionary escape paths and their impact on viral fitness allows for the development of drugs that are resistant to escape. In the case of HIV, combination...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2024
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| Online Access: | https://hdl.handle.net/1721.1/156414 |
| _version_ | 1824458062282358784 |
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| author | Galvez, Nicolas M. S. Sheehan, Maegan L. Lin, Allen Z. Cao, Yi Lam, Evan C. Jackson, Abigail M. Balazs, Alejandro B. |
| author2 | Ragon Institute of MGH, MIT and Harvard |
| author_facet | Ragon Institute of MGH, MIT and Harvard Galvez, Nicolas M. S. Sheehan, Maegan L. Lin, Allen Z. Cao, Yi Lam, Evan C. Jackson, Abigail M. Balazs, Alejandro B. |
| author_sort | Galvez, Nicolas M. S. |
| collection | MIT |
| description | Quantifying viral growth rates is key to understanding evolutionary dynamics and the potential for mutants to escape antiviral drugs. Defining evolutionary escape paths and their impact on viral fitness allows for the development of drugs that are resistant to escape. In the case of HIV, combination antiretroviral therapy can successfully prevent or treat infection, but it relies on strict adherence to prevent escape. Here, we present a method termed QuickFit that enables the quantification of viral fitness by employing large numbers of parallel viral cultures to measure growth rates accurately. QuickFit consistently recapitulated HIV growth measurements obtained by traditional approaches, but with significantly higher throughput and lower rates of error. This method represents a promising tool for rapid and consistent evaluation of viral fitness. |
| first_indexed | 2024-09-23T11:06:20Z |
| format | Article |
| id | mit-1721.1/156414 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2025-02-19T04:19:55Z |
| publishDate | 2024 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | mit-1721.1/1564142024-12-21T05:49:36Z QuickFit: A High-Throughput RT-qPCR-Based Assay to Quantify Viral Growth and Fitness In Vitro Galvez, Nicolas M. S. Sheehan, Maegan L. Lin, Allen Z. Cao, Yi Lam, Evan C. Jackson, Abigail M. Balazs, Alejandro B. Ragon Institute of MGH, MIT and Harvard Quantifying viral growth rates is key to understanding evolutionary dynamics and the potential for mutants to escape antiviral drugs. Defining evolutionary escape paths and their impact on viral fitness allows for the development of drugs that are resistant to escape. In the case of HIV, combination antiretroviral therapy can successfully prevent or treat infection, but it relies on strict adherence to prevent escape. Here, we present a method termed QuickFit that enables the quantification of viral fitness by employing large numbers of parallel viral cultures to measure growth rates accurately. QuickFit consistently recapitulated HIV growth measurements obtained by traditional approaches, but with significantly higher throughput and lower rates of error. This method represents a promising tool for rapid and consistent evaluation of viral fitness. 2024-08-28T16:14:20Z 2024-08-28T16:14:20Z 2024-08-19 2024-08-28T13:59:56Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/156414 Galvez, N.M.S.; Sheehan, M.L.; Lin, A.Z.; Cao, Y.; Lam, E.C.; Jackson, A.M.; Balazs, A.B. QuickFit: A High-Throughput RT-qPCR-Based Assay to Quantify Viral Growth and Fitness In Vitro. Viruses 2024, 16, 1320. PUBLISHER_CC http://dx.doi.org/10.3390/v16081320 Viruses Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Multidisciplinary Digital Publishing Institute Multidisciplinary Digital Publishing Institute |
| spellingShingle | Galvez, Nicolas M. S. Sheehan, Maegan L. Lin, Allen Z. Cao, Yi Lam, Evan C. Jackson, Abigail M. Balazs, Alejandro B. QuickFit: A High-Throughput RT-qPCR-Based Assay to Quantify Viral Growth and Fitness In Vitro |
| title | QuickFit: A High-Throughput RT-qPCR-Based Assay to Quantify Viral Growth and Fitness In Vitro |
| title_full | QuickFit: A High-Throughput RT-qPCR-Based Assay to Quantify Viral Growth and Fitness In Vitro |
| title_fullStr | QuickFit: A High-Throughput RT-qPCR-Based Assay to Quantify Viral Growth and Fitness In Vitro |
| title_full_unstemmed | QuickFit: A High-Throughput RT-qPCR-Based Assay to Quantify Viral Growth and Fitness In Vitro |
| title_short | QuickFit: A High-Throughput RT-qPCR-Based Assay to Quantify Viral Growth and Fitness In Vitro |
| title_sort | quickfit a high throughput rt qpcr based assay to quantify viral growth and fitness in vitro |
| url | https://hdl.handle.net/1721.1/156414 |
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