Improving electrochemical hybridization assays with restriction enzymes
Nucleic acids in blood are early indicators of disease that could be detected by point-of-care biosensors if sufficiently sensitive and facile sensors existed. Electrochemical hybridization assays are sensitive and specific but are limited to very short nucleic acids. We have developed a restriction...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Royal Society of Chemistry
2024
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| Online Access: | https://hdl.handle.net/1721.1/156724 |
| _version_ | 1811083040551600128 |
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| author | Zhou, Xingcheng Zamani, Marjon Austin, Katherine De Bock, Marieke Ullola, Joshua Chaj Rikia, Smah Furst, Ariel L. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Zhou, Xingcheng Zamani, Marjon Austin, Katherine De Bock, Marieke Ullola, Joshua Chaj Rikia, Smah Furst, Ariel L. |
| author_sort | Zhou, Xingcheng |
| collection | MIT |
| description | Nucleic acids in blood are early indicators of disease that could be detected by point-of-care biosensors if sufficiently sensitive and facile sensors existed. Electrochemical hybridization assays are sensitive and specific but are limited to very short nucleic acids. We have developed a restriction enzyme-assisted electrochemical hybridization (REH) assay for improved nucleic acid detection. By incorporating target-specific restriction enzymes, we detect long nucleic acids, with performance dependent on the location of the cut site relative to the electrode surface. Thus, we have further established guidelines for REH design to serve as a generalizable platform for robust electrochemical detection of long nucleic acids. |
| first_indexed | 2024-09-23T12:19:07Z |
| format | Article |
| id | mit-1721.1/156724 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T12:19:07Z |
| publishDate | 2024 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/1567242024-09-16T20:55:44Z Improving electrochemical hybridization assays with restriction enzymes Zhou, Xingcheng Zamani, Marjon Austin, Katherine De Bock, Marieke Ullola, Joshua Chaj Rikia, Smah Furst, Ariel L. Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Center for Environmental Health Sciences Nucleic acids in blood are early indicators of disease that could be detected by point-of-care biosensors if sufficiently sensitive and facile sensors existed. Electrochemical hybridization assays are sensitive and specific but are limited to very short nucleic acids. We have developed a restriction enzyme-assisted electrochemical hybridization (REH) assay for improved nucleic acid detection. By incorporating target-specific restriction enzymes, we detect long nucleic acids, with performance dependent on the location of the cut site relative to the electrode surface. Thus, we have further established guidelines for REH design to serve as a generalizable platform for robust electrochemical detection of long nucleic acids. 2024-09-13T15:23:26Z 2024-09-13T15:23:26Z 2024-01-29 Article http://purl.org/eprint/type/JournalArticle 1364-548X https://hdl.handle.net/1721.1/156724 Chem. Commun., 2024,60, 1948-1951 https://doi.org/10.1039/D3CC06192B ChemComm Creative Commons Attribution-Noncommercial https://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry Royal Society of Chemistry |
| spellingShingle | Zhou, Xingcheng Zamani, Marjon Austin, Katherine De Bock, Marieke Ullola, Joshua Chaj Rikia, Smah Furst, Ariel L. Improving electrochemical hybridization assays with restriction enzymes |
| title | Improving electrochemical hybridization assays with restriction enzymes |
| title_full | Improving electrochemical hybridization assays with restriction enzymes |
| title_fullStr | Improving electrochemical hybridization assays with restriction enzymes |
| title_full_unstemmed | Improving electrochemical hybridization assays with restriction enzymes |
| title_short | Improving electrochemical hybridization assays with restriction enzymes |
| title_sort | improving electrochemical hybridization assays with restriction enzymes |
| url | https://hdl.handle.net/1721.1/156724 |
| work_keys_str_mv | AT zhouxingcheng improvingelectrochemicalhybridizationassayswithrestrictionenzymes AT zamanimarjon improvingelectrochemicalhybridizationassayswithrestrictionenzymes AT austinkatherine improvingelectrochemicalhybridizationassayswithrestrictionenzymes AT debockmarieke improvingelectrochemicalhybridizationassayswithrestrictionenzymes AT ullolajoshuachaj improvingelectrochemicalhybridizationassayswithrestrictionenzymes AT rikiasmah improvingelectrochemicalhybridizationassayswithrestrictionenzymes AT furstariell improvingelectrochemicalhybridizationassayswithrestrictionenzymes |