Circular RNA migration in agarose gel electrophoresis
Circular RNAs are garnering increasing interest as potential regulatory RNAs and a format for gene expression. The characterization of circular RNA using analytical techniques commonly employed in the literature, such as gel electrophoresis, can, under differing conditions, yield different results w...
| Những tác giả chính: | , , , , |
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| Định dạng: | Bài viết |
| Ngôn ngữ: | English |
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Elsevier BV
2024
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| Truy cập trực tuyến: | https://hdl.handle.net/1721.1/156913 |
| _version_ | 1826217448518451200 |
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| author | Abe, Brian T Wesselhoeft, R Alexander Chen, Robert Anderson, Daniel G Chang, Howard Y |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Abe, Brian T Wesselhoeft, R Alexander Chen, Robert Anderson, Daniel G Chang, Howard Y |
| author_sort | Abe, Brian T |
| collection | MIT |
| description | Circular RNAs are garnering increasing interest as potential regulatory RNAs and a format for gene expression. The characterization of circular RNA using analytical techniques commonly employed in the literature, such as gel electrophoresis, can, under differing conditions, yield different results when attempting to distinguish circular RNA from linear RNA of similar molecular weights. Here, we describe circular RNA migration in different conditions, analyzed by gel electrophoresis and high-performance liquid chromatography (HPLC). We characterize key parameters that affect the migration pattern of circular RNA in gel electrophoresis systems, which include gel type, electrophoresis time, sample buffer composition, and voltage. Finally, we demonstrate the utility of orthogonal analytical tests for circular RNA that take advantage of its covalently closed structure to further distinguish circular RNA from linear RNA following in vitro synthesis. |
| first_indexed | 2024-09-23T17:03:49Z |
| format | Article |
| id | mit-1721.1/156913 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2025-02-19T04:26:23Z |
| publishDate | 2024 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1569132025-01-03T04:10:44Z Circular RNA migration in agarose gel electrophoresis Abe, Brian T Wesselhoeft, R Alexander Chen, Robert Anderson, Daniel G Chang, Howard Y Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Institute for Medical Engineering & Science Koch Institute for Integrative Cancer Research at MIT Circular RNAs are garnering increasing interest as potential regulatory RNAs and a format for gene expression. The characterization of circular RNA using analytical techniques commonly employed in the literature, such as gel electrophoresis, can, under differing conditions, yield different results when attempting to distinguish circular RNA from linear RNA of similar molecular weights. Here, we describe circular RNA migration in different conditions, analyzed by gel electrophoresis and high-performance liquid chromatography (HPLC). We characterize key parameters that affect the migration pattern of circular RNA in gel electrophoresis systems, which include gel type, electrophoresis time, sample buffer composition, and voltage. Finally, we demonstrate the utility of orthogonal analytical tests for circular RNA that take advantage of its covalently closed structure to further distinguish circular RNA from linear RNA following in vitro synthesis. 2024-09-19T21:13:30Z 2024-09-19T21:13:30Z 2022-05 2024-09-19T21:05:58Z Article http://purl.org/eprint/type/JournalArticle https://hdl.handle.net/1721.1/156913 Abe, Brian T, Wesselhoeft, R Alexander, Chen, Robert, Anderson, Daniel G and Chang, Howard Y. 2022. "Circular RNA migration in agarose gel electrophoresis." Molecular Cell, 82 (9). en 10.1016/j.molcel.2022.03.008 Molecular Cell Creative Commons Attribution https://creativecommons.org/licenses/by/4.0/ application/pdf Elsevier BV Elsevier BV |
| spellingShingle | Abe, Brian T Wesselhoeft, R Alexander Chen, Robert Anderson, Daniel G Chang, Howard Y Circular RNA migration in agarose gel electrophoresis |
| title | Circular RNA migration in agarose gel electrophoresis |
| title_full | Circular RNA migration in agarose gel electrophoresis |
| title_fullStr | Circular RNA migration in agarose gel electrophoresis |
| title_full_unstemmed | Circular RNA migration in agarose gel electrophoresis |
| title_short | Circular RNA migration in agarose gel electrophoresis |
| title_sort | circular rna migration in agarose gel electrophoresis |
| url | https://hdl.handle.net/1721.1/156913 |
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