EGFR & HER2 trafficking and signaling dynamics : experimental and modeling studies
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2003.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2005
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| Online Access: | http://hdl.handle.net/1721.1/17009 |
| _version_ | 1826192122735230976 |
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| author | Hendriks, Bart S. (Bart Sebastian), 1976- |
| author2 | Douglas A. Lauffenburger. |
| author_facet | Douglas A. Lauffenburger. Hendriks, Bart S. (Bart Sebastian), 1976- |
| author_sort | Hendriks, Bart S. (Bart Sebastian), 1976- |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2003. |
| first_indexed | 2024-09-23T09:06:36Z |
| format | Thesis |
| id | mit-1721.1/17009 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T09:06:36Z |
| publishDate | 2005 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/170092019-04-10T20:50:02Z EGFR & HER2 trafficking and signaling dynamics : experimental and modeling studies Epidermal growth factor receptor & human epidermal growth-two trafficking and signaling dynamics Hendriks, Bart S. (Bart Sebastian), 1976- Douglas A. Lauffenburger. Massachusetts Institute of Technology. Dept. of Chemical Engineering. Massachusetts Institute of Technology. Dept. of Chemical Engineering. Chemical Engineering. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2003. Includes bibliographical references. This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections. EGFR and HER2 expression levels have distinguished themselves as important factors in contributing to various types of cancers including breast and ovarian cancers, but quantitative linkages between receptor expression levels and aberrant cell behaviors are not well understood. The ability to interpret and predict cell responses in a multi-parameter space will be vital in efforts to manipulate cell behavior for therapeutic purposes. HER2 acts as a co-receptor of the EGFR family of receptor tyrosine kinases. HER2 does not bind any known ligand, but plays an active signaling role following heterodimerization with a ligand-bound EGFR family receptor. EGFR family receptors undergo a dynamic process termed trafficking in which receptors and ligands are internalized and then either recycled to the surface or targeted for degradation. Trafficking is intimately connected to cell signaling by controlling the quantity and location of ligand-receptor complexes and is sensitive to disruption via the overexpression of the receptors involved. In this work, we quantitatively establish the role of HER2 and heterodimerization in EGFR trafficking and signaling. A hierarchy of mathematical models describing the trafficking behavior of EGFR and HER2 was developed at various levels of mechanistic detail. At the macroscopic level the trafficking of EGFR and HER2 fall into two regimes, one whose downregulation is sorting-limited (EGFR) and one whose downregulation is internalization-limited (HER2). (cont.) Subordinate models yield mechanistic detail into the endocytic and endosomal sorting processes supporting the notions that heterodimers internalize as single entities and that HER2 is able to disrupt EGFR sorting through a competitive mechanism. The development of a comprehensive model of EGFR and HER2 trafficking enables the predictions of the quantity and distribution of various receptor species, including homo- and heterodimers as a function of time. Point by point comparison with ERK signaling data for different HER2 expressing cell clones allows the calculation of the signal generated per activated HER2 and per activated EGFR. These results suggest that EGFR and HER2 do not differ significantly in their ERK signaling ability and that HER2-mediated differences in ERK signaling can entirely be explained by interactions at the level of receptor trafficking. by Bart S. Hendriks. Ph.D. 2005-05-19T15:38:21Z 2005-05-19T15:38:21Z 2003 2003 Thesis http://hdl.handle.net/1721.1/17009 54454113 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 273 p. 2509833 bytes 15564805 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemical Engineering. Hendriks, Bart S. (Bart Sebastian), 1976- EGFR & HER2 trafficking and signaling dynamics : experimental and modeling studies |
| title | EGFR & HER2 trafficking and signaling dynamics : experimental and modeling studies |
| title_full | EGFR & HER2 trafficking and signaling dynamics : experimental and modeling studies |
| title_fullStr | EGFR & HER2 trafficking and signaling dynamics : experimental and modeling studies |
| title_full_unstemmed | EGFR & HER2 trafficking and signaling dynamics : experimental and modeling studies |
| title_short | EGFR & HER2 trafficking and signaling dynamics : experimental and modeling studies |
| title_sort | egfr her2 trafficking and signaling dynamics experimental and modeling studies |
| topic | Chemical Engineering. |
| url | http://hdl.handle.net/1721.1/17009 |
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