Development of in vivo Raman spectroscopy of atherosclerosis
Thesis (Ph. D.)--Harvard-MIT Division of Health Sciences and Technology, 2003.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2005
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| Online Access: | http://hdl.handle.net/1721.1/17576 |
| _version_ | 1826214609961353216 |
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| author | Motz, Jason Taylor, 1972- |
| author2 | Michael S. Feld. |
| author_facet | Michael S. Feld. Motz, Jason Taylor, 1972- |
| author_sort | Motz, Jason Taylor, 1972- |
| collection | MIT |
| description | Thesis (Ph. D.)--Harvard-MIT Division of Health Sciences and Technology, 2003. |
| first_indexed | 2024-09-23T16:08:31Z |
| format | Thesis |
| id | mit-1721.1/17576 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T16:08:31Z |
| publishDate | 2005 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/175762019-04-10T15:15:12Z Development of in vivo Raman spectroscopy of atherosclerosis Motz, Jason Taylor, 1972- Michael S. Feld. Harvard University--MIT Division of Health Sciences and Technology. Harvard University--MIT Division of Health Sciences and Technology. Harvard University--MIT Division of Health Sciences and Technology. Thesis (Ph. D.)--Harvard-MIT Division of Health Sciences and Technology, 2003. Includes bibliographical references. Cardiovascular disease is the primary cause of mortality in developed nations. Current diagnostic techniques are not able to provide the information that is related to the majority of associated complications. Raman spectroscopy, which is capable of providing a detailed chemical analysis of biological tissue, has previously been shown to be a useful method for diagnosing atherosclerosis. However, widespread clinical implementation has been prohibited by the lack of optical fiber probes which can provide remote access to small diameter vessels. To this end, we have developed a new spectroscopic model and a novel Raman probe. The model interprets Raman spectra of intact tissue in terms of its morphology. The spectrally identifiable morphological structures include collagen and elastin fibers, cholesterol crystals, calcium mineralizations, adipocytes, crystals containing 3-carotene, foam cells, necrotic core, and smooth muscle cells. This model prospectively classifies atherosclerotic tissue into three categories: non-atherosclerotic, non-calcified plaque, and calcified plaque, with >94% accuracy. Furthermore, this model has the potential ability to identify the vulnerable atherosclerotic plaques whose rupture accounts for the majority of myocardial infarctions. By studying the distribution of Raman light in tissue, we have designed, constructed, and tested small diameter, high throughput optical fiber Raman probes which employ a modular micro-optical filter configuration to remove the intense background generated in the fibers. The probes have been extensively tested during peripheral vascular surgery, providing real-time disease diagnosis for the first time. These in vivo investigations demonstrate the clinical applicability of Raman spectroscopy and have also provided the first identification of vulnerable plaques with this technique. (cont.) We have shown that plaque vulnerability is spectrally identified by determining the collagen, foam cell/necrotic core, calcification, cholesterol, and hemoglobin content of the lesion. This type of analysis may eventually lead to a diagnostic technique capable of staging atherosclerotic lesions, thereby providing a method to optimize therapeutic measures. In addition, the small diameter Raman probes have applicability for studying other diseases such as breast, oral, and gastrointestinal cancer. by Jason Taylor Motz. Ph.D. 2005-06-02T16:14:55Z 2005-06-02T16:14:55Z 2003 2003 Thesis http://hdl.handle.net/1721.1/17576 52915172 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 259 p. 12390285 bytes 12390086 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Harvard University--MIT Division of Health Sciences and Technology. Motz, Jason Taylor, 1972- Development of in vivo Raman spectroscopy of atherosclerosis |
| title | Development of in vivo Raman spectroscopy of atherosclerosis |
| title_full | Development of in vivo Raman spectroscopy of atherosclerosis |
| title_fullStr | Development of in vivo Raman spectroscopy of atherosclerosis |
| title_full_unstemmed | Development of in vivo Raman spectroscopy of atherosclerosis |
| title_short | Development of in vivo Raman spectroscopy of atherosclerosis |
| title_sort | development of in vivo raman spectroscopy of atherosclerosis |
| topic | Harvard University--MIT Division of Health Sciences and Technology. |
| url | http://hdl.handle.net/1721.1/17576 |
| work_keys_str_mv | AT motzjasontaylor1972 developmentofinvivoramanspectroscopyofatherosclerosis |