Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2003.
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| Format: | Thesis |
| Language: | en_US |
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Massachusetts Institute of Technology
2005
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| Online Access: | http://hdl.handle.net/1721.1/28286 |
| _version_ | 1826212418338947072 |
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| author | Hong, Julee Y. (Julee Yang-A.), 1980- |
| author2 | Darrell J. Irvine. |
| author_facet | Darrell J. Irvine. Hong, Julee Y. (Julee Yang-A.), 1980- |
| author_sort | Hong, Julee Y. (Julee Yang-A.), 1980- |
| collection | MIT |
| description | Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2003. |
| first_indexed | 2024-09-23T15:21:03Z |
| format | Thesis |
| id | mit-1721.1/28286 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:21:03Z |
| publishDate | 2005 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/282862019-04-10T12:19:15Z Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation Hong, Julee Y. (Julee Yang-A.), 1980- Darrell J. Irvine. Massachusetts Institute of Technology. Dept. of Materials Science and Engineering. Massachusetts Institute of Technology. Dept. of Materials Science and Engineering. Materials Science and Engineering. Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2003. Includes bibliographical references (leaf 29). The feasibility of bringing a nascent technology for detection and quantification of local cytokine concentrations on cell surfaces to market is presented in this paper. Quantum dots or fluorochrome-loaded nanoparticles are conjugated with antibodies for target analytes and with proteins that allow nanoparticle attachment to the surface of T cells. A second labeled monoclonal antibody is introduced to detect the presence of any captured-cytokines using 3D fluorescent microscopy or flow cytometry. Microscopy of DO.11 cells labeled with cytokinecapture particles have shown successful detection of exogenous IL2. A comparison of existing patents with cytokine-capture technology revealed that although each aspect of the device is covered by prior IP, the capabilities of the technology exceed the claimed uses of the individual components. A preliminary market research for cytokine-capture technology applications resulted in dismissing the immunoassay industry as a target market. However, T cell monitoring was identified as a far more lucrative industry. by Julee Y. Hong. S.B. 2005-09-26T19:34:50Z 2005-09-26T19:34:50Z 2003 2003 Thesis http://hdl.handle.net/1721.1/28286 54524845 en_US M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 29 leaves 3997127 bytes 4003410 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Materials Science and Engineering. Hong, Julee Y. (Julee Yang-A.), 1980- Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation |
| title | Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation |
| title_full | Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation |
| title_fullStr | Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation |
| title_full_unstemmed | Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation |
| title_short | Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation |
| title_sort | cytokine capture nanoparticles to quantify il 2 concentrations within the immunological synapse during t cell activation |
| topic | Materials Science and Engineering. |
| url | http://hdl.handle.net/1721.1/28286 |
| work_keys_str_mv | AT hongjuleeyjuleeyanga1980 cytokinecapturenanoparticlestoquantifyil2concentrationswithintheimmunologicalsynapseduringtcellactivation |