Extended mechanical stimulations of cartilage for growth and repair
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2004.
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| Format: | Thesis |
| Language: | en_US |
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Massachusetts Institute of Technology
2005
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| Online Access: | http://hdl.handle.net/1721.1/28445 |
| _version_ | 1811095675857797120 |
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| author | Lee, Gary C. (Gary Chiaray), 1980- |
| author2 | Alan J. Grodzinsky. |
| author_facet | Alan J. Grodzinsky. Lee, Gary C. (Gary Chiaray), 1980- |
| author_sort | Lee, Gary C. (Gary Chiaray), 1980- |
| collection | MIT |
| description | Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2004. |
| first_indexed | 2024-09-23T16:24:03Z |
| format | Thesis |
| id | mit-1721.1/28445 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:24:03Z |
| publishDate | 2005 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/284452019-04-10T14:35:12Z Extended mechanical stimulations of cartilage for growth and repair Lee, Gary C. (Gary Chiaray), 1980- Alan J. Grodzinsky. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2004. Includes bibliographical references (leaves 61-62). Extended mechanical stimulation of articular cartilage in an in vitro model explant system promotes growth and repair. An alternating day mechanical loading protocol consisting of dynamic sinusoidal compression results in long term extracelluar matrix macromolecule biosynthesis and increased biosynthetic rates in the cartilage system. The protocol uses total glycosaminoglycan and DNA content assays to measure matrix macromolecule biosynthesis and uses L-³H-proline and ³⁵S-sulfate radiolabel incorporation rates to measure rates of biosynthesis. The results also demonstrate the feasibility of replacing the fetal bovine serum and L-ascorbic acid components of the typical explant feeding medium with ITS (insulin-transferrin-selenium solution) and acorbyl-2-phosphate in anticipation of future long term studies of the synergistic pathways of dynamic compression and IGF-I stimulation for cartilage growth and repair. by Gary C. Lee. M.Eng. 2005-09-26T20:30:34Z 2005-09-26T20:30:34Z 2004 2004 Thesis http://hdl.handle.net/1721.1/28445 57015702 en_US M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 122 leaves 7302842 bytes 7317761 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science. Lee, Gary C. (Gary Chiaray), 1980- Extended mechanical stimulations of cartilage for growth and repair |
| title | Extended mechanical stimulations of cartilage for growth and repair |
| title_full | Extended mechanical stimulations of cartilage for growth and repair |
| title_fullStr | Extended mechanical stimulations of cartilage for growth and repair |
| title_full_unstemmed | Extended mechanical stimulations of cartilage for growth and repair |
| title_short | Extended mechanical stimulations of cartilage for growth and repair |
| title_sort | extended mechanical stimulations of cartilage for growth and repair |
| topic | Electrical Engineering and Computer Science. |
| url | http://hdl.handle.net/1721.1/28445 |
| work_keys_str_mv | AT leegarycgarychiaray1980 extendedmechanicalstimulationsofcartilageforgrowthandrepair |