Biological detection by means of mass reduction in a suspended microchannel resonator
Thesis (S.M.)--Massachusetts Institute of Technology, Biological Engineering Division, 2004.
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| Format: | Thesis |
| Language: | en_US |
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Massachusetts Institute of Technology
2005
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| Online Access: | http://hdl.handle.net/1721.1/28623 |
| _version_ | 1826212396140593152 |
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| author | Levy-Tzedek, Shelly |
| author2 | Scott R. Manalis. |
| author_facet | Scott R. Manalis. Levy-Tzedek, Shelly |
| author_sort | Levy-Tzedek, Shelly |
| collection | MIT |
| description | Thesis (S.M.)--Massachusetts Institute of Technology, Biological Engineering Division, 2004. |
| first_indexed | 2024-09-23T15:21:03Z |
| format | Thesis |
| id | mit-1721.1/28623 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:21:03Z |
| publishDate | 2005 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/286232019-04-11T03:21:46Z Biological detection by means of mass reduction in a suspended microchannel resonator Levy-Tzedek, Shelly Scott R. Manalis. Massachusetts Institute of Technology. Biological Engineering Division. Massachusetts Institute of Technology. Biological Engineering Division. Biological Engineering Division. Thesis (S.M.)--Massachusetts Institute of Technology, Biological Engineering Division, 2004. Includes bibliographical references (p. 33-37). Label-free detection is the detection of biomolecules and their interactions, without the use of a molecule external to the interaction, used as a reporter to indicate presence and/or location. The suspended microchannel resonator offers the opportunity to perform such label-free measurements. The goal of this work is to open new avenues of possible applications for the suspended channel. I introduce the concept of detecting mass subtraction as a new approach, rather than the conventional detection of mass addition. In a model implementation scenario of this approach, a mass-intensifying tag bound to a small ligand molecule will be equilibrated with surface-immobilized receptors, and later displaced by an identical, but label-free, ligand molecule. This approach offers opportunities to extend the sensitivity range of the device, as well as introduces new functionality for it. It enables researchers to follow, label-free, real-time enzymatic reactions, relative affinities of different ligands to a receptor, and presence of small molecules in a solution. by Shelly Levy-Tzedek. S.M. 2005-09-27T17:22:23Z 2005-09-27T17:22:23Z 2004 2004 Thesis http://hdl.handle.net/1721.1/28623 57569643 en_US MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 37 p. 2843473 bytes 2845328 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biological Engineering Division. Levy-Tzedek, Shelly Biological detection by means of mass reduction in a suspended microchannel resonator |
| title | Biological detection by means of mass reduction in a suspended microchannel resonator |
| title_full | Biological detection by means of mass reduction in a suspended microchannel resonator |
| title_fullStr | Biological detection by means of mass reduction in a suspended microchannel resonator |
| title_full_unstemmed | Biological detection by means of mass reduction in a suspended microchannel resonator |
| title_short | Biological detection by means of mass reduction in a suspended microchannel resonator |
| title_sort | biological detection by means of mass reduction in a suspended microchannel resonator |
| topic | Biological Engineering Division. |
| url | http://hdl.handle.net/1721.1/28623 |
| work_keys_str_mv | AT levytzedekshelly biologicaldetectionbymeansofmassreductioninasuspendedmicrochannelresonator |