Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, February 2005.
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| Format: | Thesis |
| Language: | en_US |
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Massachusetts Institute of Technology
2005
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| Online Access: | http://hdl.handle.net/1721.1/28936 |
| _version_ | 1811081051505688576 |
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| author | Kwiatkowski, Adam Vincent, 1974- |
| author2 | Frank Gertler. |
| author_facet | Frank Gertler. Kwiatkowski, Adam Vincent, 1974- |
| author_sort | Kwiatkowski, Adam Vincent, 1974- |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, February 2005. |
| first_indexed | 2024-09-23T11:40:58Z |
| format | Thesis |
| id | mit-1721.1/28936 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:40:58Z |
| publishDate | 2005 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/289362019-04-11T11:58:23Z Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development Kwiatkowski, Adam Vincent, 1974- Frank Gertler. Massachusetts Institute of Technology. Dept. of Biology. Massachusetts Institute of Technology. Dept. of Biology. Biology. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, February 2005. Includes bibliographical references. Regulated actin assembly drives cells movement, adhesion and shape change. The EnaNASP family of proteins controls actin filament elongation and are important regulators of axon guidance and cell motility. In vertebrates, the family consists of Mena (Mammalian Enabled), VASP (Vasodilator Stimulated Phosphoprotein), and EVL (Ena-VASP-Like). This thesis work focused on understanding the vertebrate EnaNASP protein family by discovering pathways that regulate EnaNASP function and by defining the role of EnaNASP proteins in vertebrate development. Characterization of the EVL locus revealed a new EVL isoform. A protein interaction screen for new EnaNASP ligands produced Tuba, a novel scaffold protein that associates with EnaNASP proteins in vivo. Tuba is a unique guanine nucleotide exchange factor (GEF) for Cdc42 that binds dynamin and a number of actin regulatory proteins in addition to Ena/VASP proteins. A knockout of EVL was made to determine the requirement for EVL in mouse development. Genetic analysis of EnaNASP function in the mouse revealed a requirement for Ena/VASP proteins in neuronal layering, spinal and cranial nerve formation, and cardiovascular development. by Adam Vincent Kwiatkowski. Ph.D. 2005-09-27T19:09:37Z 2005-09-27T19:09:37Z 2004 2005 Thesis http://hdl.handle.net/1721.1/28936 60552457 en_US M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 151 leaves 7733059 bytes 7753347 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biology. Kwiatkowski, Adam Vincent, 1974- Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development |
| title | Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development |
| title_full | Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development |
| title_fullStr | Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development |
| title_full_unstemmed | Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development |
| title_short | Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development |
| title_sort | characterization of tuba a novel ena vasp ligand and function of ena vasp proteins in mouse development |
| topic | Biology. |
| url | http://hdl.handle.net/1721.1/28936 |
| work_keys_str_mv | AT kwiatkowskiadamvincent1974 characterizationoftubaanovelenavaspligandandfunctionofenavaspproteinsinmousedevelopment |