Glycosaminoglycan biosynthesis controls C. elegans vulval morphogenesis and is required for the first cell division during embryogenesis
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2002.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/29740 |
| _version_ | 1826195434756898816 |
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| author | Hwang, Ho-Yon, 1969- |
| author2 | H. Robert Horvitz. |
| author_facet | H. Robert Horvitz. Hwang, Ho-Yon, 1969- |
| author_sort | Hwang, Ho-Yon, 1969- |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2002. |
| first_indexed | 2024-09-23T10:12:34Z |
| format | Thesis |
| id | mit-1721.1/29740 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T10:12:34Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/297402019-04-10T13:17:07Z Glycosaminoglycan biosynthesis controls C. elegans vulval morphogenesis and is required for the first cell division during embryogenesis Glycosaminoglycan biosynthesis controls Caenorhabditis elegans vulval morphogenesis and is required for the first cell division during embryogenesis Hwang, Ho-Yon, 1969- H. Robert Horvitz. Massachusetts Institute of Technology. Dept. of Biology. Massachusetts Institute of Technology. Dept. of Biology. Biology. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2002. Includes bibliographical references. Morphogenesis is critical to the development of multicellular organisms. Morphogenesis is also a complex process that requires the coordination of many events, including cell division, cytoskeletal reorganization, cell-cell and cell-matrix interactions. To investigate a driving mechanism of morphogenesis in organ formation, we characterized a group of C. elegans mutants defective in vulval morphogenesis. We established that the morphogenetic defect is caused by disruption in biosynthesis of chondroitin glycosaminoglycans by molecular identification and characterization of five sqv (uashed vulva) genes. Glycosaminoglycans are known to function in development by binding a large repertoire of ligands involved in morphogenesis and wound healing, in some cases by modulating certain conserved signaling pathways, and in providing structural support in the extracellular matrix. Defects in biosynthesis of glycosaminoglycans can cause connective tissue diseases. We found that regulation of sqv-4 likely controls glycosaminoglycan biosynthesis and the aspect of vulval morphogenesis driven by chondroitin. We also characterized specific defects in separation of the plasma membrane from the eggshell and failure to initiate cytokinesis caused by mutations in sqv genes in the one-cell embryo. by Ho-Yon Hwang. Ph.D. 2006-03-24T16:21:03Z 2006-03-24T16:21:03Z 2002 2002 Thesis http://hdl.handle.net/1721.1/29740 54052632 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 202 leaves 9967380 bytes 9967186 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biology. Hwang, Ho-Yon, 1969- Glycosaminoglycan biosynthesis controls C. elegans vulval morphogenesis and is required for the first cell division during embryogenesis |
| title | Glycosaminoglycan biosynthesis controls C. elegans vulval morphogenesis and is required for the first cell division during embryogenesis |
| title_full | Glycosaminoglycan biosynthesis controls C. elegans vulval morphogenesis and is required for the first cell division during embryogenesis |
| title_fullStr | Glycosaminoglycan biosynthesis controls C. elegans vulval morphogenesis and is required for the first cell division during embryogenesis |
| title_full_unstemmed | Glycosaminoglycan biosynthesis controls C. elegans vulval morphogenesis and is required for the first cell division during embryogenesis |
| title_short | Glycosaminoglycan biosynthesis controls C. elegans vulval morphogenesis and is required for the first cell division during embryogenesis |
| title_sort | glycosaminoglycan biosynthesis controls c elegans vulval morphogenesis and is required for the first cell division during embryogenesis |
| topic | Biology. |
| url | http://hdl.handle.net/1721.1/29740 |
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