Biophysical characterization of high affinity engineered single chain Fv antibody fragments

Biophysical characterization of high affinity engineered single chain Fv antibody fragments

Thesis (Ph. D. in Molecular Systems Toxicology and Pharmacology)--Massachusetts Institute of Technology, Biological Engineering Division, 2004.

Bibliographic Details
Main Author:	Midelfort, Katarina Senn
Other Authors:	K. Dane Wittrup.
Format:	Thesis
Language:	eng
Published:	Massachusetts Institute of Technology 2006
Subjects:	Biological Engineering Division.
Online Access:	http://hdl.handle.net/1721.1/30051

Similar Items

Characterization of Single-Chain Fv Fragments of Neutralizing Antibodies to Rabies Virus Glycoprotein
by: Kohei Yumoto, et al.
Published: (2021-11-01)

A central composite design to investigate antibody fragment production by Pichia pastoris
by: Chan, Joyce, M. Eng. Massachusetts Institute of Technology
Published: (2006)

Expression, production, and renaturation of a functional single-chain variable antibody fragment (scFv) against human ICAM-1
by: H. Sun, et al.
Published: (2014-07-01)

Isolation of antigen-specific intracellular antibody fragments as single chain Fv for use in mammalian cells.
by: Tse, E, et al.
Published: (2002)

Mechanisms of Action and Limitations of Monoclonal Antibodies and Single Chain Fragment Variable (scFv) in the Treatment of Cancer
by: Cynthia Rodríguez-Nava, et al.
Published: (2023-06-01)

Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C
by: O. S. Oliinyk, et al.
Published: (2015-04-01)

Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein
by: De Santis Rita, et al.
Published: (2006-02-01)

Computational modeling of protein-biomolecule interactions with application to mechanotransduction and antibody maturation
by: Zyto, Aurore
Published: (2009)

Development of a filter device for the prevention of aquatic bacterial disease using a single-chain variable fragment (scFv)-conjugated affinity silk
by: Harsha Prakash, et al.
Published: (2022-06-01)

Comparison of the growth and monoclonal antibody production of suspended mammalian cells in three perfusion systems
by: Hufford, Kathy (Kathy E.)
Published: (2008)

Biodegradable microfluidic scaffolds for vascular tissue engineering
by: Bettinger, Christopher John, 1981-
Published: (2005)

Exploring the mechanome with optical tweezers and single molecule fluorescence
by: Brau, Ricardo R. (Ricardo Rafael), 1979-
Published: (2008)

Physicochemical characterization of immortal strand DNA
by: Lansita, Janice A. (Janice Ann), 1975-
Published: (2005)

Nanoscale and microscale approaches for engineering the in vitro cellular microenvironment
by: Khademhosseini, Ali
Published: (2006)

Applying engineering principles to the design and construction of transcriptional devices
by: Shetty, Reshma P. (Reshma Padmini)
Published: (2009)

Genetic engineering of bacteriophage and its applications for biomimetic materials
by: Lee, Soo-Kwan
Published: (2007)

Engineering phosphorylation-dependent post-translational protein devices
by: Sutton, Samantha C. (Samantha Carol)
Published: (2009)

Engineering the interface between cellular chassis and synthetic biological systems
by: Canton, Bartholomew (Bartholomew John)
Published: (2009)

Quantitative analysis and characterization of intracellular gene delivery mechanisms
by: Varga, Csanad M. (Csanad Mathias), 1976-
Published: (2005)

Mitotic homologous recombination at engineered repeats in S. cerevisiae and in novel transgenic mice
by: Hendricks, Carrie A. (Carrie Anne), 1975-
Published: (2006)

Development of an ostrich-derived single-chain variable fragment (scFv) against PTPRN extracellular domain
by: Hamed Dabiri, et al.
Published: (2024-02-01)

Development of simple 3D-printed scaffolds for liver tissue engineering
by: Camp, James (James Patrick), 1977-
Published: (2005)

Tools and reference standards supporting the engineering and evolution of synthetic biological systems
by: Kelly, Jason R. (Jason Robert)
Published: (2009)

A directed evolution approach to engineering recombinant protein production in S. cerevisiae
by: Rakestraw, James A
Published: (2007)

CONSTRUCTION AND BACTERIAL EXPRESSION OF AN ACTIVE SINGLE-CHAIN FV ANTIBODY SPECIFIC FOR SCORPION HEMOCYANINS
by: Billiald, P, et al.
Published: (1994)

Use of gene expression to characterize heterogeneous liver cell populations
by: Schreiber, Brent M. (Brent Matthew), 1981-
Published: (2005)

Computational structure-based modeling and analysis with application to rational and evolutionary molecular engineering
by: Armstrong, Kathryn Anne
Published: (2008)

The impact of age, exposure and genetics on homologous recombination at the engineered repeat sequence in mice
by: Wiktor-Brown, Dominika M
Published: (2008)

Characterization of self-assembling peptide nanofibers of KLD12 and RID 12
by: Dai, Jessica, 1981-
Published: (2005)

Enzymatic and analytical tools for the characterization of chondroitin sulfate and dermatan sulfate glycosaminoglycans
by: Pojasek, Kevin R. (Kevin Robert), 1976-
Published: (2005)

Therapeutics discovery via glycotechnology : the development of protein engineered enzymes for the study of galactosaminoglycan neuromedicine
by: Prabhakar, Vikas
Published: (2008)

Mapping the actin and actin binding proteins interactions : from micromechanics to single molecule force spectroscopy
by: Ferrer, Jorge M., 1976-
Published: (2008)

Using optical tweezers, single molecule fluorescence and the ZIF268 protein-DNA system to probe mechanotransduction mechanisms
by: Lee, Peter, S.M. Massachusetts Institute of Technology
Published: (2006)

Development, characterization and transcriptional profiling of a mouse model of fatal infectious diarrhea and colitis
by: Borenshtein, Diana
Published: (2008)

Fundamental differentiation and growth characterization of murine embryonic stem cells in varied culture conditions
by: Hashimura, Yasunori, 1980-
Published: (2005)

Development and characterization of an in vitro culture system as a physiological model for chronic Hepatitis B infection
by: Sams, Alexandria V. (Alexandria Victoria)
Published: (2008)

Detection and characterization of rat hepatic stellate cells in a 3-dimensional, perfused, liver bioreactor
by: Wack, Kathryn E. (Kathryn Eilleen), 1978-
Published: (2005)

Synthesis of a soluble flag-tagged single chain variable fragment (scFv) antibody targeting Cucumber Mosaic Virus (CMV) coat protein
by: Chua, K.H., et al.
Published: (2003)

The characterization of obesity and noninsulin dependent diabetes mellitus in Swiss Webster mice associated with late-onset hepatocellular carcinoma
by: Lemke, Laura B. (Laura Beth)
Published: (2009)

Correction: Development of Phage-Based Single Chain Fv Antibody Reagents for Detection of Yersinia pestis
Published: (2012-01-01)