Regulation and production of extracellular signaling molecules in Bacillus subtilis
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2004.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/30068 |
| _version_ | 1826210403823124480 |
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| author | McQuade, Ryan S. (Ryan Scott), 1975- |
| author2 | Alan D. Grossman. |
| author_facet | Alan D. Grossman. McQuade, Ryan S. (Ryan Scott), 1975- |
| author_sort | McQuade, Ryan S. (Ryan Scott), 1975- |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2004. |
| first_indexed | 2024-09-23T14:49:15Z |
| format | Thesis |
| id | mit-1721.1/30068 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T14:49:15Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/300682019-04-12T09:41:05Z Regulation and production of extracellular signaling molecules in Bacillus subtilis McQuade, Ryan S. (Ryan Scott), 1975- Alan D. Grossman. Massachusetts Institute of Technology. Dept. of Biology. Massachusetts Institute of Technology. Dept. of Biology. Biology. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2004. Includes bibliographical references. Many bacteria use chemical signals for intercellular communication. These signals accumulate extracellularly and are sensed at threshold concentrations to alter gene expression. Bacillus subtilis uses cell-cell signals to control genetic competence, sporulation, degradative enzyme production and antibiotic synthesis. A family of peptide signals controls gene expression in B. subtilis by regulating transcription factors. I showed that the transcription of these peptide regulators is in turn controlled in part by the alternate sigma factor sigma-H. The activity of sigma-H is stimulated by nutrient starvation I determined that B. subtilis produces a putative interspecies signaling activity, AI-2, that depends on the conserved protein LuxS. To determine the effect of luxS on regulation of transcription in B. subtilis, I performed experiments to compare the transcriptional profiles of B. subtilis when luxS was deleted or overexpressed. I found no significant change in transcriptional profiles, indicating that B. subtilis does not use luxS/AI-2 for signaling under these conditions. by Ryan S. McQuade. Ph.D. 2006-03-24T18:16:37Z 2006-03-24T18:16:37Z 2004 2004 Thesis http://hdl.handle.net/1721.1/30068 55634684 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 120 leaves 5727882 bytes 5727689 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biology. McQuade, Ryan S. (Ryan Scott), 1975- Regulation and production of extracellular signaling molecules in Bacillus subtilis |
| title | Regulation and production of extracellular signaling molecules in Bacillus subtilis |
| title_full | Regulation and production of extracellular signaling molecules in Bacillus subtilis |
| title_fullStr | Regulation and production of extracellular signaling molecules in Bacillus subtilis |
| title_full_unstemmed | Regulation and production of extracellular signaling molecules in Bacillus subtilis |
| title_short | Regulation and production of extracellular signaling molecules in Bacillus subtilis |
| title_sort | regulation and production of extracellular signaling molecules in bacillus subtilis |
| topic | Biology. |
| url | http://hdl.handle.net/1721.1/30068 |
| work_keys_str_mv | AT mcquaderyansryanscott1975 regulationandproductionofextracellularsignalingmoleculesinbacillussubtilis |