Modeling and measurement of intermolecular interaction forces between cartilage ECM macromolecules
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2005.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/30153 |
| _version_ | 1826212638555635712 |
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| author | Dean, Delphine Marguerite Denise, 1978- |
| author2 | Alan J. Grodzinsky. |
| author_facet | Alan J. Grodzinsky. Dean, Delphine Marguerite Denise, 1978- |
| author_sort | Dean, Delphine Marguerite Denise, 1978- |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2005. |
| first_indexed | 2024-09-23T15:30:17Z |
| format | Thesis |
| id | mit-1721.1/30153 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T15:30:17Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/301532019-04-12T14:11:36Z Modeling and measurement of intermolecular interaction forces between cartilage ECM macromolecules Dean, Delphine Marguerite Denise, 1978- Alan J. Grodzinsky. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2005. Includes bibliographical references (p. 143-151). The mechanical properties of cartilage tissue depend largely on the macromolecules that make up its extracellular matrix (ECM). Aggrecan is the most abundant proteoglycan in articular cartilage. It is composed of a core protein with highly charged, densely packed glycosaminoglycan (GAG) side chains, which are responsible for [approximately] 50% of the equilibrium compressive stiffness of the tissue. Using atomic force microscopy (AFM) and high resolution force spectroscopy (HRFS), it is now possible to directly measure nanoscale interactions between ECM macromolecules in physiologically relevant aqueous solution conditions. In order to interpret these data and compare them to macroscopic tissue measurements, a combination of experiments and theoretical modeling must be used. In this thesis, a new molecular-scale continuum Poisson-Boltzmann (PB)-based model was developed to predict the intermolecular interactions between GAG macromolecules by taking into account nanoscale space varying electric potential and fields between neighboring GAGs. A rod-like charge density distribution describing the time averaged space occupied by a single GAG chain was formulated. The spacing and size of the rods greatly influenced the calculated force even when the total charge was kept constant. The theoretical simulations described HRFS experimental data of the normal interaction force between two surfaces chemically end-grafted with an array of GAGs ("brushes") more accurately than simpler models which approximate the GAG charge as a homogeneous volume or planar surface charge. Taken together, these results highlight the importance of nonuniform molecular-level charge distribution on the measured GAG interaction forces. Normal interaction forces between aggrecan macromolecules were measured using contact mode AFM imaging and by HRFS. (cont.) The aggrecan molecules were end-grafted to gold-coated substrates and probe tips to achieve brush-like layers at physiologically relevant densities. Both colloidal probe tips (2.5[micro]m radius) and sharper probe tips ([approximately] 25-50nm radius) were used. The measured normal forces were predominantly repulsive and showed a strong ionic strength dependence reflecting the importance of repulsive electrostatic interactions. These aggrecan-aggrecan forces were much larger than those previously measured between brushes composed only of a single layer of GAG chains isolated from aggrecan molecules. The measured aggrecan normal force interactions were then compared to the predictions of the PB charged rod model for GAG electrostatic interactions and to measurements of the equilibrium compressive modulus of intact cartilage tissue. At near physiological bath conditions (0.1M NaCl), the PB electrostatic model closely predicted the values of the measured force for nanomechanical strains < 0.4, using model parameter values that were all fixed to their known values from the literature. At higher strains, the measured normal forces were higher than those predicted by the model, qualitatively consistent with the likelihood that other nonelectrostatic interactions were becoming more important. A compressive stiffness was also calculated from the measured aggrecan-aggrecan nanomechanical force data, and was found to be [approximately] 50% of the modulus of native intact cartilage. This is consistent with previous reports suggesting that aggrecan-associated electrostatic interactions account for approximately half of the tissue modulus. by Delphine Marguerite Denise Dean. Ph.D. 2006-03-24T18:24:24Z 2006-03-24T18:24:24Z 2005 2005 Thesis http://hdl.handle.net/1721.1/30153 60654182 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 151 p. 8276401 bytes 8296751 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science. Dean, Delphine Marguerite Denise, 1978- Modeling and measurement of intermolecular interaction forces between cartilage ECM macromolecules |
| title | Modeling and measurement of intermolecular interaction forces between cartilage ECM macromolecules |
| title_full | Modeling and measurement of intermolecular interaction forces between cartilage ECM macromolecules |
| title_fullStr | Modeling and measurement of intermolecular interaction forces between cartilage ECM macromolecules |
| title_full_unstemmed | Modeling and measurement of intermolecular interaction forces between cartilage ECM macromolecules |
| title_short | Modeling and measurement of intermolecular interaction forces between cartilage ECM macromolecules |
| title_sort | modeling and measurement of intermolecular interaction forces between cartilage ecm macromolecules |
| topic | Electrical Engineering and Computer Science. |
| url | http://hdl.handle.net/1721.1/30153 |
| work_keys_str_mv | AT deandelphinemargueritedenise1978 modelingandmeasurementofintermolecularinteractionforcesbetweencartilageecmmacromolecules |