Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly
In the post genomic era, proteomics has enormous potential in biology and medicine. Among the various bioanalytical tools developed, protein microarray is one of the recent advancements which offer high throughput profiling of cellular proteins to provide insights into the mechanisms of biological p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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2005
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| Online Access: | http://hdl.handle.net/1721.1/30384 |
| _version_ | 1826193319081803776 |
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| author | Ajikumar, Parayil Kumaran Ng, Jin Kiat Tang, Yew Chung Lee, Jim Yang Stephanopoulos, Gregory Too, Heng-Phon |
| author_facet | Ajikumar, Parayil Kumaran Ng, Jin Kiat Tang, Yew Chung Lee, Jim Yang Stephanopoulos, Gregory Too, Heng-Phon |
| author_sort | Ajikumar, Parayil Kumaran |
| collection | MIT |
| description | In the post genomic era, proteomics has enormous potential in biology and medicine. Among the various bioanalytical tools developed, protein microarray is one of the recent advancements which offer high throughput profiling of cellular proteins to provide insights into the mechanisms of biological processes. Fundamentally, the protein microarray involves the immobilization of interacting elements, proteins, on a few square microns of a solid support and in principle, it is capable of detecting analytes with a higher sensitivity than conventional macroscopic immunoassays. Here in the present report we delineates the design, fabrication and functional analysis of protein microarray using semi-synthetic ssDNA tagged-proteins as capturing moiety as well as address on a solid support. Optimization of the platform has been carried out by investigating various parameters such as surface chemistry, signal amplification, and conditions for homogenous liguid phase protein-protein interaction. |
| first_indexed | 2024-09-23T09:37:05Z |
| format | Article |
| id | mit-1721.1/30384 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T09:37:05Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | mit-1721.1/303842019-04-12T07:24:08Z Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly Ajikumar, Parayil Kumaran Ng, Jin Kiat Tang, Yew Chung Lee, Jim Yang Stephanopoulos, Gregory Too, Heng-Phon Proteomics Protein micro array Dendrimer glass slide ssDNA-antibody In the post genomic era, proteomics has enormous potential in biology and medicine. Among the various bioanalytical tools developed, protein microarray is one of the recent advancements which offer high throughput profiling of cellular proteins to provide insights into the mechanisms of biological processes. Fundamentally, the protein microarray involves the immobilization of interacting elements, proteins, on a few square microns of a solid support and in principle, it is capable of detecting analytes with a higher sensitivity than conventional macroscopic immunoassays. Here in the present report we delineates the design, fabrication and functional analysis of protein microarray using semi-synthetic ssDNA tagged-proteins as capturing moiety as well as address on a solid support. Optimization of the platform has been carried out by investigating various parameters such as surface chemistry, signal amplification, and conditions for homogenous liguid phase protein-protein interaction. Singapore-MIT Alliance (SMA) 2005-12-16T14:42:45Z 2005-12-16T14:42:45Z 2006-01 Article http://hdl.handle.net/1721.1/30384 en Molecular Engineering of Biological and Chemical Systems (MEBCS) 131181 bytes application/pdf application/pdf |
| spellingShingle | Proteomics Protein micro array Dendrimer glass slide ssDNA-antibody Ajikumar, Parayil Kumaran Ng, Jin Kiat Tang, Yew Chung Lee, Jim Yang Stephanopoulos, Gregory Too, Heng-Phon Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly |
| title | Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly |
| title_full | Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly |
| title_fullStr | Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly |
| title_full_unstemmed | Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly |
| title_short | Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly |
| title_sort | design fabrication and functional analysis of a new protein array based on ssdna based assembly |
| topic | Proteomics Protein micro array Dendrimer glass slide ssDNA-antibody |
| url | http://hdl.handle.net/1721.1/30384 |
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