Development of biodegradable microfluidic networks for tissue engineering
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2002.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/32238 |
| _version_ | 1811093072761585664 |
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| author | King, Kevin R. (Kevin Robert), 1976- |
| author2 | Joel Voldman, Jeffrey T. Borenstein and Joseph P. Vacanti. |
| author_facet | Joel Voldman, Jeffrey T. Borenstein and Joseph P. Vacanti. King, Kevin R. (Kevin Robert), 1976- |
| author_sort | King, Kevin R. (Kevin Robert), 1976- |
| collection | MIT |
| description | Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2002. |
| first_indexed | 2024-09-23T15:38:42Z |
| format | Thesis |
| id | mit-1721.1/32238 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T15:38:42Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/322382019-04-11T09:38:39Z Development of biodegradable microfluidic networks for tissue engineering King, Kevin R. (Kevin Robert), 1976- Joel Voldman, Jeffrey T. Borenstein and Joseph P. Vacanti. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2002. Includes bibliographical references. Biodegradable tissue engineering scaffolds currently suffer from poorly controlled geometries, lack of reproducibility, and severe mass transport limitations. Microfabrication is an ideal tool for attacking problems in tissue engineering due to its control of diverse size scales, from microns to centimeters, all with micron resolution and submicron precision. By enabling precision geometries and flexible designs, microfabrication has the potential to offer creative solutions to some of the major problems facing the field of tissue engineering; those of achieving rapid vascularization and recapitulating normal complex tissue microarchitecture. In this thesis, silicon micromachining, soft lithography, and traditional polymer processing are combined to develop a fully biodegradable microfabrication platform using poly(DL-lactic-co-glycolic) acid (PLGA 85:15). First, micron scale structures fabricated on silicon substrates are transferred into the surface of biodegradable films using polymer melt replica molding. Next, microchannel networks are sealed and made three-dimensional by stacking and irreversibly bonding the biodegradable films using a newly developed thermal fusion bonding process. The process is modeled and optimized to guide bonding of a wide range of microchannels while avoiding parasitic microstructure deformation. An extruded inlet/outlet scheme is implemented and combined with micromolding and fusion bonding to build fully patent, leak-free biodegradable microfluidic networks with predictable fluidic resistances. Finally, this thesis concludes with the first demonstration of long-term continuous-flow cell culture in prototype microfluidic networks, demonstrating the feasibility of using the newly developed biodegradable microdevices as cell-seeded tissue engineering scaffolds. In comparison to conventional scaffold fabrication techniques, these processes offer two orders of magnitude improvement in fabrication time and spatial resolution while offering flexible designs and unmatched reproducibility. Through these features, they have enabled construction of the first fully biodegradable microfluidic networks. by Kevin R. King. S.M. 2006-03-29T18:26:46Z 2006-03-29T18:26:46Z 2002 2002 Thesis http://hdl.handle.net/1721.1/32238 51977771 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 155 p. 19134299 bytes 19685940 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science. King, Kevin R. (Kevin Robert), 1976- Development of biodegradable microfluidic networks for tissue engineering |
| title | Development of biodegradable microfluidic networks for tissue engineering |
| title_full | Development of biodegradable microfluidic networks for tissue engineering |
| title_fullStr | Development of biodegradable microfluidic networks for tissue engineering |
| title_full_unstemmed | Development of biodegradable microfluidic networks for tissue engineering |
| title_short | Development of biodegradable microfluidic networks for tissue engineering |
| title_sort | development of biodegradable microfluidic networks for tissue engineering |
| topic | Electrical Engineering and Computer Science. |
| url | http://hdl.handle.net/1721.1/32238 |
| work_keys_str_mv | AT kingkevinrkevinrobert1976 developmentofbiodegradablemicrofluidicnetworksfortissueengineering |