Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2004.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/32256 |
| _version_ | 1826199027217072128 |
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| author | Lee, Janice Ying, 1974- |
| author2 | Terry L. Orr-Weaver. |
| author_facet | Terry L. Orr-Weaver. Lee, Janice Ying, 1974- |
| author_sort | Lee, Janice Ying, 1974- |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2004. |
| first_indexed | 2024-09-23T11:13:34Z |
| format | Thesis |
| id | mit-1721.1/32256 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T11:13:34Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/322562019-04-12T09:55:07Z Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila Lee, Janice Ying, 1974- Terry L. Orr-Weaver. Massachusetts Institute of Technology. Dept. of Biology. Massachusetts Institute of Technology. Dept. of Biology. Biology. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2004. Includes bibliographical references. In cell division, the proper segregation of chromosomes requires sister-chromatid cohesion. This physical attachment between sister chromatids is established during DNA replication, maintained throughout mitosis and released at the metaphase-anaphase transition. In meiosis, sister-chromatid cohesion is released along the chromosome arms in the first meiotic division, but retained at the centromere until the second meiotic division. In this thesis, we have analyzed the localization of two cohesion proteins in Drosophila, MEI-S332 and RAD21. MEI-S332 localizes specifically to the centromere from prometaphase to the metaphase-anaphase transition in mitosis, and from prometaphase I to the metaphase II-anaphase II transition in meiosis. We find that the termini of MEI-S332 are required for its localization to chromosomes; these are also the regions that have homology to MEI-S332-like proteins in other organisms. The localization of MEI-S332 does not require the presence of cohesin, an evolutionarily conserved protein complex that is essential for the establishment and maintenance of cohesion, nor a replicated sister chromatid. However, MEI-S332 delocalization is dependent upon the activity of the separase pathway that regulates cohesin release. We have identified and characterized a key subunit of cohesin in Drosophila, DRAD21, and studied its localization in early stages of meiosis in spermatocytes. (cont.) DRAD21 is nuclear in prophase I, but is not visibly localized on chromosomes in later stages. Although DRAD21 is concentrated in centromeric regions after prometaphase in mitosis, MEI- S332 and DRAD21 do not physically interact in a complex in whole embryo extracts. Immunostaining of spread metaphase chromosomes for MEI-S332 and DRAD21 reveals that the two proteins are not localized to the same domains. by Janice Ying Lee. Ph.D. 2006-03-29T18:28:14Z 2006-03-29T18:28:14Z 2004 2004 Thesis http://hdl.handle.net/1721.1/32256 56023866 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 243 leaves 7707729 bytes 7704094 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biology. Lee, Janice Ying, 1974- Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila |
| title | Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila |
| title_full | Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila |
| title_fullStr | Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila |
| title_full_unstemmed | Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila |
| title_short | Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila |
| title_sort | localization studies of sister chromatid cohesion proteins mei s332 and rad21 in drosophila |
| topic | Biology. |
| url | http://hdl.handle.net/1721.1/32256 |
| work_keys_str_mv | AT leejaniceying1974 localizationstudiesofsisterchromatidcohesionproteinsmeis332andrad21indrosophila |