A study of peptide affinity for carbon nanotubes through phage display experiments
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2004.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/32265 |
| _version_ | 1811085860730306560 |
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| author | Lau, Caroline Kar Yan, 1978- |
| author2 | Yet-Ming Chiang. |
| author_facet | Yet-Ming Chiang. Lau, Caroline Kar Yan, 1978- |
| author_sort | Lau, Caroline Kar Yan, 1978- |
| collection | MIT |
| description | Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2004. |
| first_indexed | 2024-09-23T13:16:41Z |
| format | Thesis |
| id | mit-1721.1/32265 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T13:16:41Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/322652019-04-12T09:09:19Z A study of peptide affinity for carbon nanotubes through phage display experiments Lau, Caroline Kar Yan, 1978- Yet-Ming Chiang. Massachusetts Institute of Technology. Dept. of Materials Science and Engineering. Massachusetts Institute of Technology. Dept. of Materials Science and Engineering. Materials Science and Engineering. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2004. Includes bibliographical references (p. 55-60). The use of biological molecules to facilitate the dispersal, separation and assembly of nanoscopic entities such as carbon nanotubes has received great attention and has been the focus of much current research activity. In this work we sought to identify peptide sequences with selective affinity for HiPco-produced SWNTs in order to gain some insight to the binding mechanisms and interactions. This was done using a phage display technique, in which a library of bacteriophages displaying greater than 109 different 12-amino acid long peptide sequences were exposed to carbon nanotubes. Non-specifically bound phages were successively washed off with increasingly stronger detergents until only tightly binding phages remained. It was observed that after six rounds of phage display tests, the percentage of sites of aromatic ring-containing amino acids increased while the percentage of sites of aliphatic amino acids decreased. Results were compared to previous phage display results on MWNTs. These results suggest that peptides are able to distinguish between different allotropes of carbon and that their highly specific binding mechanisms can be exploited in the future for use in precision placement of nanoscale components in devices such as electronic circuits and sensors. by Caroline Kar Yan Lau. S.M. 2006-03-29T18:29:00Z 2006-03-29T18:29:00Z 2004 2004 Thesis http://hdl.handle.net/1721.1/32265 56028885 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 60 p. 1828519 bytes 1827000 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Materials Science and Engineering. Lau, Caroline Kar Yan, 1978- A study of peptide affinity for carbon nanotubes through phage display experiments |
| title | A study of peptide affinity for carbon nanotubes through phage display experiments |
| title_full | A study of peptide affinity for carbon nanotubes through phage display experiments |
| title_fullStr | A study of peptide affinity for carbon nanotubes through phage display experiments |
| title_full_unstemmed | A study of peptide affinity for carbon nanotubes through phage display experiments |
| title_short | A study of peptide affinity for carbon nanotubes through phage display experiments |
| title_sort | study of peptide affinity for carbon nanotubes through phage display experiments |
| topic | Materials Science and Engineering. |
| url | http://hdl.handle.net/1721.1/32265 |
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