A New Biobrick Assembly Strategy Designed for Facile Protein Engineering

The existing biobrick assembly technique[1] provides a straightforward way to combine standardized biological components, termed biobricks. This system, however, is limited in that each protein-encoding biobrick must contain a complete translated region. Signal sequences or other protein domains...

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Bibliographic Details
Main Authors: Phillips, Ira, Silver, Pamela
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering
Format: Technical Report
Language:en_US
Published: 2006
Subjects:
Online Access:http://hdl.handle.net/1721.1/32535
Description
Summary:The existing biobrick assembly technique[1] provides a straightforward way to combine standardized biological components, termed biobricks. This system, however, is limited in that each protein-encoding biobrick must contain a complete translated region. Signal sequences or other protein domains often convey a specific function to the protein to which they are attached; hence, each domain should be considered an independent biological part. With the current assembly technique, assembling such parts is not possible. This paper presents a revised assembly strategy that is compatible with the current biobrick definition and permits the construction of fusion proteins.