The effects of membrane and cytoskeletal mechanics on cell adhesiveness
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004.
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
| Published: |
Massachusetts Institute of Technology
2006
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/32785 |
| _version_ | 1826195846168838144 |
|---|---|
| author | Shearer, David, 1982- |
| author2 | Richard J. Gilbert. |
| author_facet | Richard J. Gilbert. Shearer, David, 1982- |
| author_sort | Shearer, David, 1982- |
| collection | MIT |
| description | Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004. |
| first_indexed | 2024-09-23T10:16:28Z |
| format | Thesis |
| id | mit-1721.1/32785 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T10:16:28Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/327852019-04-10T09:16:01Z The effects of membrane and cytoskeletal mechanics on cell adhesiveness Shearer, David, 1982- Richard J. Gilbert. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Mechanical Engineering. Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004. Includes bibliographical references (leaves 28-29). The cytoskeleton, the internal network of filaments that regulates cell shape and structure, has been implicated in several critical aspects of cell adhesion. Its role, however, has primarily been demonstrated as a component of the numerous intracellular signaling events that regulate cell surface receptors. In this study we sought to gain insight into how the mechanical properties of the cytoskeleton could affect adhesion. Specifically, we addressed how membrane deformability, which we demonstrate is critically dependent upon tethering to the cytoskeleton, could impact a whole cell adhesion assay. To modify membrane deformability, we treated cells with the pharmacological agents phalloidin, Latrinculin B, Cytochalasin D, colchicine, and Paclitaxel, which have varying effects on microfilaments and microtubules, two of the main constituents of the cytoskeleton. We found that dissolution of the actin cytoskeleton could reduce the number of adherent cells to a callogen-coated substrate by over 85%. We theorize that this is due largely to the inhibition of signaling events associated with the cytoskeleton, but it may also be affected by changes in shape and deformability. To truly understand the implications of this experiment, we believe further study using high-resolution force technology such as atomic force microscopy or magnetic bead microrheometry is necessary. by David Shearer. S.B. 2006-05-15T20:29:58Z 2006-05-15T20:29:58Z 2004 2004 Thesis http://hdl.handle.net/1721.1/32785 57582935 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 29 leaves 1507256 bytes 1505937 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Shearer, David, 1982- The effects of membrane and cytoskeletal mechanics on cell adhesiveness |
| title | The effects of membrane and cytoskeletal mechanics on cell adhesiveness |
| title_full | The effects of membrane and cytoskeletal mechanics on cell adhesiveness |
| title_fullStr | The effects of membrane and cytoskeletal mechanics on cell adhesiveness |
| title_full_unstemmed | The effects of membrane and cytoskeletal mechanics on cell adhesiveness |
| title_short | The effects of membrane and cytoskeletal mechanics on cell adhesiveness |
| title_sort | effects of membrane and cytoskeletal mechanics on cell adhesiveness |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/32785 |
| work_keys_str_mv | AT shearerdavid1982 theeffectsofmembraneandcytoskeletalmechanicsoncelladhesiveness AT shearerdavid1982 effectsofmembraneandcytoskeletalmechanicsoncelladhesiveness |