Antibody-Antigen assay design for combined optical tweezers and fluorescence
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/32789 |
| _version_ | 1811093211105460224 |
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| author | Ta, Jenny, 1982- |
| author2 | Matthew J. Lang. |
| author_facet | Matthew J. Lang. Ta, Jenny, 1982- |
| author_sort | Ta, Jenny, 1982- |
| collection | MIT |
| description | Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004. |
| first_indexed | 2024-09-23T15:41:25Z |
| format | Thesis |
| id | mit-1721.1/32789 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T15:41:25Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/327892019-04-12T09:48:43Z Antibody-Antigen assay design for combined optical tweezers and fluorescence Ta, Jenny, 1982- Matthew J. Lang. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Mechanical Engineering. Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004. Includes bibliographical references (p. 37). The recent development in combined optical trapping and fluorescence technology promises to enable unbindinig force studies of receptor-ligand interactions, whose specificity play a crucial role in the function of many biological systems. This thesis focuses on the development of assay designs for the study of antibody-antigen binding interactions using combined optical trapping and single molecule fluorescence. The assays create the necessary linkage geometry between the antibody-antigen system under study to an optically trapped bead, enabling force probing of the antibody-antigen binding interaction. In particular, two tether materials and fluorophores were studied: polyethylene glycol (PEG) with Cy-2, and dsDNA with fluorescein. We demonstrate tether formation in the dsDNA-fluorescein antibody-antigen linkage system with preliminary optical trapping data. by Jenny Ta. S.B. 2006-05-15T20:30:14Z 2006-05-15T20:30:14Z 2004 2004 Thesis http://hdl.handle.net/1721.1/32789 57583131 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 37 p. 1177088 bytes 1176599 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Ta, Jenny, 1982- Antibody-Antigen assay design for combined optical tweezers and fluorescence |
| title | Antibody-Antigen assay design for combined optical tweezers and fluorescence |
| title_full | Antibody-Antigen assay design for combined optical tweezers and fluorescence |
| title_fullStr | Antibody-Antigen assay design for combined optical tweezers and fluorescence |
| title_full_unstemmed | Antibody-Antigen assay design for combined optical tweezers and fluorescence |
| title_short | Antibody-Antigen assay design for combined optical tweezers and fluorescence |
| title_sort | antibody antigen assay design for combined optical tweezers and fluorescence |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/32789 |
| work_keys_str_mv | AT tajenny1982 antibodyantigenassaydesignforcombinedopticaltweezersandfluorescence |