Design and characterization of a gel loading mechanism for an ultra-high throughput mutational spectrometer
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005.
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| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/32881 |
| _version_ | 1826208398659551232 |
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| author | Ball, Nathan B |
| author2 | Ian W. Hunter. |
| author_facet | Ian W. Hunter. Ball, Nathan B |
| author_sort | Ball, Nathan B |
| collection | MIT |
| description | Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005. |
| first_indexed | 2024-09-23T14:05:09Z |
| format | Thesis |
| id | mit-1721.1/32881 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T14:05:09Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/328812019-04-10T11:14:05Z Design and characterization of a gel loading mechanism for an ultra-high throughput mutational spectrometer Ball, Nathan B Ian W. Hunter. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Mechanical Engineering. Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005. Includes bibliographical references (leaf 51). A process known as Constant Denaturant Capillary Electrophoresis is used to separate mutant from wild-type DNA at fractions down to 10-7. A device known as an Ultra-high Throughput Mutational Spectrometer is being created to run 10,000 parallel channels of CDCE in order to correlate multiple point mutations in DNA with the diseases that they can cause, such as cancer. By separating the DNA in large populations, the underlying causes of such diseases can be identified. To successfully run CDCE, a high viscosity polymer gel must be loaded into each of the 10,000 channels, each of which are composed of an individual glass capillary with a 75 m inner diameter. A mechanism was designed and tested which loaded gel into 8 channels simultaneously. The mechanism was used to test the relationship between gel loading time in relation to varied pressure and capillary length, through 45 total runs, with 8 channels per run. The relationships were characterized, resulting in two equations that enable an accurate prediction of the fill time necessary to load 10,000 parallel channels simultaneously under varied conditions. by Nathan B. Ball. S.B. 2006-05-15T20:36:41Z 2006-05-15T20:36:41Z 2005 2005 Thesis http://hdl.handle.net/1721.1/32881 62589055 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 73 leaves 2890463 bytes 2893211 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Ball, Nathan B Design and characterization of a gel loading mechanism for an ultra-high throughput mutational spectrometer |
| title | Design and characterization of a gel loading mechanism for an ultra-high throughput mutational spectrometer |
| title_full | Design and characterization of a gel loading mechanism for an ultra-high throughput mutational spectrometer |
| title_fullStr | Design and characterization of a gel loading mechanism for an ultra-high throughput mutational spectrometer |
| title_full_unstemmed | Design and characterization of a gel loading mechanism for an ultra-high throughput mutational spectrometer |
| title_short | Design and characterization of a gel loading mechanism for an ultra-high throughput mutational spectrometer |
| title_sort | design and characterization of a gel loading mechanism for an ultra high throughput mutational spectrometer |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/32881 |
| work_keys_str_mv | AT ballnathanb designandcharacterizationofagelloadingmechanismforanultrahighthroughputmutationalspectrometer |