Temperature response of the ultra-high throughput mutational spectrometer
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/32960 |
| _version_ | 1811094570903011328 |
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| author | Suen, Timothy W. (Timothy Wu) |
| author2 | Ian W. Hunter. |
| author_facet | Ian W. Hunter. Suen, Timothy W. (Timothy Wu) |
| author_sort | Suen, Timothy W. (Timothy Wu) |
| collection | MIT |
| description | Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005. |
| first_indexed | 2024-09-23T16:02:11Z |
| format | Thesis |
| id | mit-1721.1/32960 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T16:02:11Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/329602019-04-10T08:07:00Z Temperature response of the ultra-high throughput mutational spectrometer Suen, Timothy W. (Timothy Wu) Ian W. Hunter. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Mechanical Engineering. Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005. Includes bibliographical references (p. 43-44). The Ultra-High Throughput Mutational Spectrometer is an instrument designed to separate mutant from wild type DNA through capillary electrophoresis. Since this technique uses the melting point of the molecule to distinguish between sequences of base pairs, temperature control is crucial to the success of the device. The purpose of this analysis is to characterize the temperature response of the instrument, taking into account the heat dissipated by the 10,000 capillaries in the system during electrophoresis. Analytical models, finite element analysis, and physical models were used to predict the steady state response of the system to heat generated by capillary electrophoresis. The analytical models estimated a steady state offset of 0.2 K for water at 3.3x 10Ì⁴ m³/s (20 L/min) and 1.0 K for water at 6.7x 10Ì⁵ m³/s (4.0 L/min) and predicted that the system would reach steady state within several seconds. Finite element analysis determined that the gel inside the capillaries would have a steady state offset of 0.24 K. The physical system, which simulated the Joule heating of the capillaries using an immersion heater, yielded a steady state offset of 0.24 K at 3.3x 10Ì⁴ m³/s and 0.65 K at 6.7x 10Ì⁵ m³/s, but the settling time in both cases was on the order of 500 s. (cont.) This discrepancy is due to the fact that many aspects of the physical system, including the thermal mass of the instrument, heat loss through convection, and the PID temperature controller in the circulator, were not taken into consideration in the theoretical analysis. Pressure drop and vortex shedding were also calculated for the instrument. Finite element analysis determined the pressure drop to be 18.55 Pa. Vortex shedding does not occur, because the operating conditions of this instrument are below the critical Reynolds number. by Timothy W. Suen. S.B. 2006-05-15T20:42:05Z 2006-05-15T20:42:05Z 2005 2005 Thesis http://hdl.handle.net/1721.1/32960 62859714 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 44 p. 2627636 bytes 2627602 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Suen, Timothy W. (Timothy Wu) Temperature response of the ultra-high throughput mutational spectrometer |
| title | Temperature response of the ultra-high throughput mutational spectrometer |
| title_full | Temperature response of the ultra-high throughput mutational spectrometer |
| title_fullStr | Temperature response of the ultra-high throughput mutational spectrometer |
| title_full_unstemmed | Temperature response of the ultra-high throughput mutational spectrometer |
| title_short | Temperature response of the ultra-high throughput mutational spectrometer |
| title_sort | temperature response of the ultra high throughput mutational spectrometer |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/32960 |
| work_keys_str_mv | AT suentimothywtimothywu temperatureresponseoftheultrahighthroughputmutationalspectrometer |