Characterization of human skin emanations by Solid Phase Microextraction (SPME) extraction of volatiles and subsequent analysis by Gas Chromatography-Mass Spectrometry (GC-MS)
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2005.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/33399 |
| _version_ | 1826213115082047488 |
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| author | Akin James (James J.) |
| author2 | Christina E. Davis and Darrell J. Irvine. |
| author_facet | Christina E. Davis and Darrell J. Irvine. Akin James (James J.) |
| author_sort | Akin James (James J.) |
| collection | MIT |
| description | Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2005. |
| first_indexed | 2024-09-23T15:43:51Z |
| format | Thesis |
| id | mit-1721.1/33399 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T15:43:51Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/333992019-04-11T05:17:54Z Characterization of human skin emanations by Solid Phase Microextraction (SPME) extraction of volatiles and subsequent analysis by Gas Chromatography-Mass Spectrometry (GC-MS) Akin James (James J.) Christina E. Davis and Darrell J. Irvine. Massachusetts Institute of Technology. Dept. of Materials Science and Engineering. Massachusetts Institute of Technology. Dept. of Materials Science and Engineering. Materials Science and Engineering. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2005. Includes bibliographical references (p. 80-82). An experimental study was performed to develop and validate a collection and analysis protocol for human skin emanations. The protocol developed included the rubbing of glass beads on the palms and backs of hands for 20 minutes. The volatile headspace above samples were extracted by a solid-phase microextraction fiber which incorporated a composite coating of liquid polymer matrix and solid porous particles. This protocol provided robust and convenient signatures of human skin emanations and was applied to two experiments for validation. In one experiment, a set of twins donated samples and results suggested qualitative differences between samples of twins. The second experiment involved collections from four unrelated individuals over a period of one month. Multivariate analysis was applied to this data set and indicated a stable signature that can be ascribed to the individual, confirming that the protocol developed here can be extended to larger sample sets of MHC typed individuals. by James Akin. S.M. 2006-07-13T15:21:04Z 2006-07-13T15:21:04Z 2005 2005 Thesis http://hdl.handle.net/1721.1/33399 62708596 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 99 p. 4559379 bytes 4563473 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Materials Science and Engineering. Akin James (James J.) Characterization of human skin emanations by Solid Phase Microextraction (SPME) extraction of volatiles and subsequent analysis by Gas Chromatography-Mass Spectrometry (GC-MS) |
| title | Characterization of human skin emanations by Solid Phase Microextraction (SPME) extraction of volatiles and subsequent analysis by Gas Chromatography-Mass Spectrometry (GC-MS) |
| title_full | Characterization of human skin emanations by Solid Phase Microextraction (SPME) extraction of volatiles and subsequent analysis by Gas Chromatography-Mass Spectrometry (GC-MS) |
| title_fullStr | Characterization of human skin emanations by Solid Phase Microextraction (SPME) extraction of volatiles and subsequent analysis by Gas Chromatography-Mass Spectrometry (GC-MS) |
| title_full_unstemmed | Characterization of human skin emanations by Solid Phase Microextraction (SPME) extraction of volatiles and subsequent analysis by Gas Chromatography-Mass Spectrometry (GC-MS) |
| title_short | Characterization of human skin emanations by Solid Phase Microextraction (SPME) extraction of volatiles and subsequent analysis by Gas Chromatography-Mass Spectrometry (GC-MS) |
| title_sort | characterization of human skin emanations by solid phase microextraction spme extraction of volatiles and subsequent analysis by gas chromatography mass spectrometry gc ms |
| topic | Materials Science and Engineering. |
| url | http://hdl.handle.net/1721.1/33399 |
| work_keys_str_mv | AT akinjamesjamesj characterizationofhumanskinemanationsbysolidphasemicroextractionspmeextractionofvolatilesandsubsequentanalysisbygaschromatographymassspectrometrygcms |