Characterization of human expired breath by solid phase microextraction and analysis using gas chromatography-mass spectrometry and differential mobility spectrometry
Thesis (M. Eng.)--Harvard-MIT Division of Health Sciences and Technology, 2005.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2006
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| Online Access: | http://hdl.handle.net/1721.1/33847 |
| _version_ | 1811089389828177920 |
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| author | Merrick, William (William F. W.) |
| author2 | Cristina E. Davis and Julie E. Greenberg. |
| author_facet | Cristina E. Davis and Julie E. Greenberg. Merrick, William (William F. W.) |
| author_sort | Merrick, William (William F. W.) |
| collection | MIT |
| description | Thesis (M. Eng.)--Harvard-MIT Division of Health Sciences and Technology, 2005. |
| first_indexed | 2024-09-23T14:18:24Z |
| format | Thesis |
| id | mit-1721.1/33847 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T14:18:24Z |
| publishDate | 2006 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/338472019-04-11T09:08:21Z Characterization of human expired breath by solid phase microextraction and analysis using gas chromatography-mass spectrometry and differential mobility spectrometry Merrick, William (William F. W.) Cristina E. Davis and Julie E. Greenberg. Harvard University--MIT Division of Health Sciences and Technology. Harvard University--MIT Division of Health Sciences and Technology. Harvard University--MIT Division of Health Sciences and Technology. Thesis (M. Eng.)--Harvard-MIT Division of Health Sciences and Technology, 2005. Includes bibliographical references (leaves 92-95). Breath analysis has potential to become a new medical diagnostic modality. In this thesis, a method for the analysis of human expired breath was developed using gas chromatography-mass spectroscopy. It was subsequently adopted for gas chromatography-differential mobility spectroscopy, a modality not previously applied to this problem. Tedlar bags and solid-phase microextraction were used for breath sampling and concentration prior to analysis. Four fiber coatings were evaluated with respect to selectivity and sensitivity; extraction time, gas chromatography temperature programming, and sample storage stability were explored for optimization. The method entails extraction and preconcentration with a polydimethylsiloxane-divinylbenzene coated fiber for 30 min at 37⁰C, and extraction profiles for several compounds demonstrate competitive adsorption. 120 compounds were identified in breath with response variability between 23 - 117% about mean values. Feasibility of differential mobility spectroscopy for breath analysis was established, and this method will be the basis for future investigations on the diagnostic potential of breath analysis. by William Merrick. M.Eng. 2006-08-25T18:50:39Z 2006-08-25T18:50:39Z 2005 2005 Thesis http://hdl.handle.net/1721.1/33847 65466497 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 95 leaves 4181829 bytes 4185745 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Harvard University--MIT Division of Health Sciences and Technology. Merrick, William (William F. W.) Characterization of human expired breath by solid phase microextraction and analysis using gas chromatography-mass spectrometry and differential mobility spectrometry |
| title | Characterization of human expired breath by solid phase microextraction and analysis using gas chromatography-mass spectrometry and differential mobility spectrometry |
| title_full | Characterization of human expired breath by solid phase microextraction and analysis using gas chromatography-mass spectrometry and differential mobility spectrometry |
| title_fullStr | Characterization of human expired breath by solid phase microextraction and analysis using gas chromatography-mass spectrometry and differential mobility spectrometry |
| title_full_unstemmed | Characterization of human expired breath by solid phase microextraction and analysis using gas chromatography-mass spectrometry and differential mobility spectrometry |
| title_short | Characterization of human expired breath by solid phase microextraction and analysis using gas chromatography-mass spectrometry and differential mobility spectrometry |
| title_sort | characterization of human expired breath by solid phase microextraction and analysis using gas chromatography mass spectrometry and differential mobility spectrometry |
| topic | Harvard University--MIT Division of Health Sciences and Technology. |
| url | http://hdl.handle.net/1721.1/33847 |
| work_keys_str_mv | AT merrickwilliamwilliamfw characterizationofhumanexpiredbreathbysolidphasemicroextractionandanalysisusinggaschromatographymassspectrometryanddifferentialmobilityspectrometry |